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BB guy 1
bb guy section 1 notes from start through ABO
Question | Answer |
---|---|
Low Ionic Strength Saline (LISS) enhances these antibodies | cold antibodies and autoantibodies |
Polyethylene glycol (PEG) enhances these antibodies | warm antibodies and autoantibodies |
Immediate spin (IS) phase antibodies are usually this isotype | IgM |
This test detects RBCs coated with IgG +/– complement in vivo | DAT (Direct Coombs) |
Explain the term "dosage" in regards to RBC antigens | Some antibodies react more strongly with RBCs that have double-dose (“homozygous”) antigen expression |
enzymes like papain and ficin decrease the reactions associated with these RBC antigens | MNS System Duffy System |
enzymes like papain and ficin increase the reactions associated with these RBC antigens | UCLA Pneumonic: Lewis P Is A Rhotten Kidd" ABO-related: ABO, H Systems, Lewis System, I System, P System Rh System Kidd System |
The effect of enzymes on the Kell blood group antigens when performing antibody identification | None |
substance used to neutralize ABO antibodies | ABO: Saliva (secretor) |
substance used to neutralize Lewis antibodies | Saliva (secretor for Leb) |
substance used to neutralize P1 antibodies | Hydatid cyst fluid Pigeon/dove egg white |
substance used to neutralize Sda antibodies | Human urine |
substance used to neutralize Chido antibodies | Serum (complement) |
substance used to neutralize Rodgers antibodies | Serum (complement) |
Specificity of the lectin Dolichos biflorus | A1 |
Specificity of the lectin Ulex europaeus | H (absent in Bombay phenotype) |
Specificity of the lectin Vicia graminea | N |
Specificity of the lectin Arachis hypogea | T |
Specificity of the lectin Glycine max | T, Tn |
Specificity of the lectin Salvia | Tn |
Most significant antibodies are this isotype and react best at this temperature | IgG and "warm reactive" |
Most insignificant antibodies are this isotype and react best at this temperature | IgM and “cold reactive |
Se (“secretor”) gene codes for this enzyme | FUT2; “fucosyluridyl transferase” FUT enzyme adds fucose to type 1 chains at terminal galactose; product is type 1 H antigen |
This gene is required to make A or B antigens in secretions | Se gene |
Frequency of Se gene | 80% |
this antigen is required before A and/or B can be made on RBCs | H type 2 H on RBCs or in secretions, type 1 H |
This sugar is added to a type 2 H antigen chain to make Group A RBCs | N-acetylgalactosamine |
This sugar is added to a type 2 H antigen chain to make Group B RBCs | Galactose |
ABO antigens begin to appear on fetal RBCs at ___ weeks gestation | 6 |
ABO antigens are also present on these tissues | platelets, endothelium, kidney, heart, lung, bowel, pancreas tissue |
Isotype of antibodies to ABO antigens in group O people | IgG |
Most common ABO group in USA | O>A>B>AB |
Why is HDFN not severe in type O mom with type A baby? | Weak fetal ABH expression, soluble ABH antigens (neutralize antibodies) |
amount of A antigen present on A1 RBCs compared to A2 RBCs | 5x |
The cause of acquired B phenotype | Bacterial enzymes (deacetylase) deacetylate group A GalNAc; remaining galactosamine looks like B |
Conditions associated with acquired B phenotype | Colon cancer, intestinal obstruction, gram-negative sepsis |
Naturally occurring antibodies in the Bombay phenotype | anti-A, anti-B, anti-AB, anti-H Total lack of H, A and B antigens due to lack of H and Se genes (genotype: hh, sese) |
Para-Bombay phenotype differs from Bombay phenotype because of this | have at least one Se gene RBCs may be Bombay-like, but may also show free or RBC A or B antigens (unless group O) Antibody profile is same as Bombay |