Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Enzymes 2
| Question | Answer |
|---|---|
| This enzyme phosphorylates phosphorylase in glycogenolysis | Phosphorylase kinase |
| Elevated cAMP binds to this enzyme and removes the inhibitory subunit | Protein Kinase A |
| Hydrolyzes glycogen | Phosphorylase |
| Remove phosphates on phosphorylase kinase and phsophorylase | Protein phsophatases |
| Activates IP3, DAG and Ca2+ | Phospholipase C |
| Stimulated by diacylglycerol (DAG) and inositol trisphosphate 3 (IP3) | Protein kinase C |
| Cleaves phosphodiester bonds | Nuclease |
| A nuclease that cleaves at the end of a DNA strand and releases an dNMP | Exonuclease |
| Hydrolyze nucleotides at either the 3' end or 5' end | Exonuclease |
| Hydrolyze nucleotides at the 3' end | 3'->5' exonuclease |
| Hydrolyze nucleotides at the 5' end | 5'->3' exonuclease |
| Hydrolyze the phsophodiester bond in the middle of a DNA strand | Endonucleases |
| Restriction enzymes are what kind of enzyme? | Endonucleases |
| Forms a phosphodiester bond between the 3' OH and 5' phosphate | DNA ligase |
| Unwinds DNA at the replication fork in E. coli | Helicase |
| Adds negative supercoils ahead of replication fork in E. coli | DNA gyrase |
| Synthesizes RNA Primer in E. coli | Primase |
| Synthesizes the bulk of the DNA in E. coli | DNA pol III |
| Removes RNA primers and fills in the gaps in E. coli | DNA pol I |
| Sealse the nicks in E. coli | DNA ligase |
| Requires a primer | DNA polymerase |
| Responsible for priming synthesis in human cells | DNA polymerase alpha |
| Synthesizes the bulk of both strands of DNA in human cells | DNA polymerase delta |
| Synthesizes a short stretch of RNA and then extens this RNA in human cells to prime replication | DNA polymerase alpha |
| Remove RNA and any incorrect DNA bases misincorporated by pol alpha in eukaryotic cells | RNAse H1 and Flap endonuclease-1 (FEN-1) |
| Move along the DNA backbone and scan for the presence of damaged bases | N-glycosidases and AP endonucleases |
| Recognizes the absent base and clips the phosphodiester backbone on the 5' side of the AP site | AP endonuclease |
| Recognizes AP sites that are created by ROS or formed spontaneously | AP endonuclease |
| Scan by "base flipping" | N-glycosidases |
| Removes a base if it recognizes damage to the base while "base flipping" | N-glycosidases |
| Synthesizes RNA from a DNA template | |
| Requires a DNA template, rNTPs and Mg++ | RNA polymerase |
| Shaped like a "crab claw" with the acitve site located at the base of the groove between the two "pincers" | RNA polymerase |
| Maintain negative supercoiling so that polymerase can move along the DNA strand in transcription | Topoisomerases |
| Transcribes ribosomal RNA genes (with the exception of 5S RNA) in eukaryotes | RNA pol I |
| Transcribes mRNAs and some small RNAs involved in splicing in eukaryotes | RNA pol II |
| Transcribes tRNAs, 5S RNA and various small RNAs involved in splicing and RNA processing in eukaryotes | RNA pol III |
| Forms a peptide bond between the P site peptide (carboxyl group) and the A site amino acid (NH3 group) | Peptidyltransferase |
| Rate-limiting step of the Pentose Phosphate Pathway | Glucose-6-P Dehydrogenase (GPDH) |
| Rate-limiting step of the glycolysis | Phosphofructokinase |
| Allow for interconversion of diasteromers | Epimerase |
| Transfers a 3-carbon fragment from one sugar to another | Transaldolase |
| Transfers a 2-carbon frament from one sugar to another | Transketolase |
| Makes citrate from OAA and acetyl-CoA | Citrate synthase |
| Reduces OAA to malate | Malate dehydrogenase |
| Decarboxylates malate to pyruvate and generates NADPH | Malic enzyme |
| Rate-limiting step of fatty acid synthesis (requires biotin) | Acetyl CoA carboxylase |
| Presence of these enzymes in the liver are indicative of liver disease | Alanine aminotransferase (ALP/SGPT) and Aspartate aminotransferase (AST/SGOT) |
| Increased levels of this enzyme in the blood is indicative of heart disease or myocardial infaction | Creatine kinase |
| Fluorouracil is a suicide inhibitor of this enzyme | Thymidylate synthase |
Created by:
P1StudyStack
Popular Biochemistry sets