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VA Microbiology
VTA 130 Microbiology
| Question | Answer |
|---|---|
| list the three basic shapes of bacteria | coccus (round), Bacillus (rods), spirillum (spirals) |
| Gram negative bacteria stain is what color | pink |
| Gram positive bacteria stain is what color | purple |
| what is the purpose of a gram stain | to differentiate between gram positive and gram negative bacteria |
| what are the 3 pieces of information we label on a culture plate | client name, date, where swab came from |
| how is a culture plate placed in the incubator | upside down |
| what are the three types of hemolysis | alpha, beta, gamma |
| name two types of stain used in microbiology | New Methylene blue, Gram |
| define morphology | the description of size, shape, color |
| what is the scientific name for canine ear yeast | Malassezia pachydermatis |
| what is DTM | drematified testing media |
| describe alpha hemolysis | green/brown incomplete hemolysis |
| describe beta hemolysis | complete hemolysis |
| describe gama hemolysis | no color, no hemolysis |
| what are the Gram stains used in order (CIDS) | crystal violet, iodine, decolorize, safranin |
| what is the first thing you do in the Gram stain procedure | heat fix |
| what are the types of media | basic, enriched, selective, differential, Mueller-Hinton Agar, |
| what is enriched media enriched with | 5% sheep's blood |
| what is hemolysis | blood destruction |
| what is the first lab rule | assume all cultures are bio hazardous waste |
| name the three basic shapes of bacteria | cocci (round), bacillus (rods), sprillium (spiral) |
| what do we do use a gram stain for | it is the first step in identifying a bacterial organism or pathogen |
| what is the first step we do when doing a gram stain | heat fix it |
| what are the stains we use in a gram stain | crystal violet, iodine, decolorer, safranin |
| how long do we allow the decolorer stain to stay on | 10 sec |
| why do we do a gram stain | to differentiate between gram positive and gram negative bacteria |
| what is formites | inanament objects that transfer bacteria |
| what is nosocomial | acquiring bacteria from a health care facility |
| what is an aerobic specimen | requires air to live |
| what is an anaerobic specimen | can't survive in oxygen |
| what are the six types of media | basic, enriched, selective, differential, Mueller-Hinton agar, broth |
| what is hemolysis | blood destruction |
| what is morphology | size, shape, color, etc |
| what is the BAP | blood auger plate |
| what is the enriched media enriched with | 5% sheeps blood |
| what is DTM | dramatophyte testing media |
| What temp is the BAP incubated at | 37 degrees C = 98.6 degrees F/ body temp |
| what is a DTM incubated at | room temperature |
| to inoculate our BAP plate we need what | gloves, sterile swab, media, incubator |
| what do we use the Mueller-Hinton auger used for | for antibiotic susceptibility testing |
| what will grow lime green under the woods lamp | ring worm |
| what is a drematafite | ring worm |
| what is mycology | study of fungus |
| what does canine ear yeast look like under the microscope | like a footprint or a kidney bean |
Created by:
fitnessgal
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