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Yeast

Yeast and Dimorphic Pathogens

QuestionAnswer
Which organism has the unique property of producing germ tubes when fetal bovine serum is lightly inoculated with the yeast isolate and incubated at 35 - 37° C for 2.5 - 3 hours? Candida albicans
On cornmeal agar: produces pseudohyphae, regular clusters of blastoconidia along the points of septation, and large, spherical chlamydospores Candida albicans
On cornmeal agar: has terminal chlamydospores that occur in pairs or clusters Candia dubliniensis
On cornmeal agar: characterized by true hyphae that segment into rectangular arthroconidia. Arthrospores germinate from one corner, giving them a "hockey stick" appearance Geotrichum sp.
Does not produce pseudohyphae. It reproduces by successively budding, producing multilateral, oval yeast cells that may form short chains. It is usually pink to coral in color on cornmeal agar. Rhodotorula rubra
Oval to round yeast cells with multilateral budding. Ascopores may be seen when ascospore or Kinyoun stain is used. Saccharomyces
Produce both hyphae and psuedohyphae. Blastoconidia are present. Trichosporon
"Logd in a stream" blastoconidia Candida kefyr
"Hockey stick" arthroconidia Geotrichum sp.
Multilateral budding yeast cells Rhodotorula rubra
Mucoid colonies. Urease positive. Reddish-brown pigmentation on bird seed agar. Presence of capsule. No pseudohyphae on cornmeal agar. Cryptococcus neoformans
produces the enzyme phenoloxidase, which oxidizes the caffein acid in bird seed agar to melanin, producing a red-brown pigmentation Cryptococcus neoformans
Associated with a catheter- either an indwelling intravenous catheter, an intraventricular shunt or the catheter used in continuous peritoneal dialysis. Rhodotorula rubra
Fungi that have a yeast (or yeast-like) phase and a mold (filamentous) phase. Dimorphic fungi
Production of mold form at 25-30C and yeast form at 35-37C Dimorphic fungi
Dimorphic fungi that are considered pathogens Blastomyces dermatitidis Histoplasma capsulatum Coccidioides immitis Paracoccidioides brasilliensis Sporothric schenckii
Mold form: Spiked (echinulate) macroconidia Histoplasma capsulatum
Mold form: Production of arthroconidia that appear alternate staining. When mature, each arthroconidium is swollen centrally, giving a barrel appearance. Coccidioides immitis
Mold form: Produce spherical or oval conida, each supported by an individual conidiophore (lollipop) Blastomyces dermatitidis
Mold form: Production of tiny microconidia that are arranged around the tip of a delicate conidiophore as petals on a daisy flower. Sporothrix schenckii
Yeast form: large, 10-15 um in diameter, thick-walled yeast cells that produces a single bud attached by a broad base. Blastomyces dermatitidis
Yeast form: does not produce a yeast form in a laboratory culture. Identified in stained tissue sections by the production of varying sized spherules. The larger, more mature spherules endospores ranging from 2-4um in diameter. Coccidioides immitis
Yeast form: produces large yeast cells with multiple buds attached by narrow necks, giving the appearance of a "mariner's wheel" Paracoccidioides brasiliensis
Yeast form: elongated cells that have been celled "cigar bodies" Sporothrix schenckii
Systemic disease, mostly of the reticuloendothelial system. It may manifest itself in the bone marrow, lungs, liver, and/or spleen. The primary indication of infection in children is hepatosplenomegaly. In adults, it most often occurs as pulmonary disease Histoplasmosis
Transmitted through the guano of bats and birds, particularly chicken and blackbirds. Contracted by individuals who frequent caves in endemic areas such as KY and states in the lower Missouri and Mississippi river valley Histoplasmosis
Pulmonary disease that is contracted from inhalation of wind blown spores in dust; near the burrows of desert rodents. Coccidiodomycosis
Referred to as "rose grower's disease". Sportrichosis
Enter the body through a thorn prick. A pustule develops and ulcerates. Infects the lymphatic system. Sporothrix schenckii
referred to as "Chicago disease". Can be acquired by close contact with dogs or through scratches and bites. Blastomycosis
Presents as a cutaneous or a respiratory disease Blastomycosis
Affects skin, mucous membranes, and the pulmonary system. Commonly found in South America, Central America, and Africa. Present in soil in the endemic areas and contracted when eating vegetation that was grown in that soil Paracoccidioidomycosis
Gray-white and delicate cob-web appearance on blood agar. Histoplasma capsulatum
Gray-white and have a delicate hair-like consistency on blood agar after 8 days of incubation at 30C Coccidioides immitis
Gray-green, delicate, cottony consistency on Sabourand's dextrose agar. Sporothric schenckii
Although care should be taken when working with all fungus cultures in the laboratory, personnel are particularly prone to develop laboratory acquired infections from the inhalation of airborne species of which pathogenic fungi? Coccidioides immitis
Which dimorphic fungus may produce black, yeast-like colonies after prolonged incubation at 37°C? Sporothrix schenckii
Which ingredient is added to culture media to enhance the recovery of the dimorphic fungi by preventing the overgrowth of more rapidly growing, saprophytic molds? Cycloheximide
What is often used as an enrichment to enhance the primary recovery of dimorphic fungi from clinical materials; however, it also enhances the growth of other fungal species as well. Brain heart infusion
What is added to culture media to prevent the overgrowth of bacteria? Chloramphenicol
An enrichment needed for the growth of a few select fungal species; notably, the dermatophyte Trichophyton tonsurans. It has little influence on the growth of the dimorphic fungi Thiamine
Created by: emery87