click below
click below
Normal Size Small Size show me how
Practicum I
Review of Labs 1-6
Term | Definition |
---|---|
Isolation | Uses four quadrants for individual colonies (Ex: throat, wound) |
Quantitation | Method uses calibrated .001 loop Also you has one central streak, then spread out in 2 directions |
Stab and Streak | used with slant media in tube |
Obligate Aerobe | grow on top or "slant" part of media |
Obligate anaerobe | will grow in bottom or "butt" part of media |
Facultative anaerobe | will grow on both the slant and butt |
MacConkey | selective for G- and differential based on fermentation of lactose |
Pink color on MacConkey plate agar indicate to | Lactose fermentation (E |
Clear colonies on MacConkey plate agar indicates to | non-lactose fermenter |
BAP agar plate is made of | 5% sheep blood |
BAP | Supportive for most organisms except fastidious |
BAP is differential based on types of | hemolysis |
Beta hemolysis | complete clear zone |
Alpha hemolysis | partial hemolysis, green zone around colonies |
Gamma hemolysis | no hemolysis or no zone around colonies |
EMB is selective for what type of microorganism and differential based for | Gram negative, lactose fermentation |
Metallic green sheen indicates for ... on EMB | lactose fermenter |
Clear colonies on EMB indicate | non-lactose fermenter |
Mannitol Salt Agar it is ... | selective and differential media |
MSA is selective for ... microorganism that appear ... color upon its mannitol use | halophilic, yellow |
Orange/ red color on MSA media indicates to a | negative fermenter |
Specimen in broth that is used in KB Disk Diffusion it is | 0.5 McFarland standard |
Zone of inhibition is measured in | mm (millimeters) |
Primary stain | Crystal violet |
Iodine | mordant |
Safranin | counter stain |
Decolorizer | alcohol or acetone based |
G+ organism | purple/blue |
G- organism | pink/red |
Spherical and blue organisms are.... | G+ cocci |
Rod shaped and red | G- bacilli |
Pivotal test for G+ cocci | Catalase |
Reagent used in Catalase test is | Hydrogen peroxide |
S.aureus, S.epidermidis, and M.luteus are positive to this test | Catalase test |
Optochin test is a presumptive test that is used to identify | S.pneumonia |
Rabbit plasma is a reagent in the what kind of test? | Coagulase test |
What letter is printed on Optichin disk? | P |
what kind of organism is sensitive to Optichin Disk? | S.pneumonia |
Bacitracin disk test | used as presumprive ID of Group A Steptococci |