click below
click below
Normal Size Small Size show me how
Enzymology
WVSOM Class of 2012 Enzymology
| Question | Answer |
|---|---|
| neg. delta G leads to what kind of reaction? | favorable |
| do enzymes change the delta G of a reaction? | no |
| at what point in a reaction do substrates have their highest energy level? | transition point |
| what must happen to glucose before it can enter a cell? | hexokinase must phosphorylate it |
| why isn't galactose a good substrate? | bad stereochemistry |
| in acid-base catalysis, which amino acid side chain is most likely used? | histidine |
| what is the optimum ph range for chymotrypsin? | above the pka |
| aside from histidine, what protease uses acid-base catalysis? | aspartic proteases |
| where are aspartic proteases found in cells? | lysosomes |
| give an example of an oxidoreductase? | ADH |
| what does an activation transfer coenzyme do? | transfers functional groups from one molecule to another |
| NAD and FAD are examples of what? | redox coenzymes |
| NAD is synthesized from what vitamin? | niacin |
| FAD is synthesized from what vitamin? | riboflavin |
| is NAD a coenzyme or a substrate? | both |
| what is important for maintaining redox balance? | glutathione (reduced) |
| give examples of activation transfer coenzymes: | pyrophosphate, coa, biotin, pyridoxal phosphate |
| what reaction type is FAD? NAD? | both are redox reactions |
| TPP is what vitamin? | thiamine (B1) |
| CoA is what vitamin? | pantothenate (B5) |
| what is km? | concentration of substrate needed to reach 1/2 vmax |
| would competitive inhibition affect km or vmax? | km |
| in noncompetitive inhibition, what is changed? | vmax |
| what kind of molecule acts at a site other than the active site? | allosteric |
| noncompetitive inhibition does or does not alter the reaction km? | does not |
| what inhibition is frequently seen in multisubstrate enzymes? | noncompetitive inhibition |
| which inhibition is rare? what is changed | uncompetitive inhibition, km and vmax |
| give an example of nonspecific enzyme inhibition: | nonoptimal ph, temperature |