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NOV BRDS

QuestionAnswer
IgG vs. IgM IgG vs. IgM
Structure of IgG monomer
structure of IgM pentamer
number of binding sites IgG=2 igM=10
type of antibody of IgG immune
type of antibody of IgM natural occurring
Optimum temp of reactivity IgG 37
ABO DISCREPENCIES ABO DISCREPENCIES
anti-A, Anti B, A1, A2, B cells, O cells,auto 0 0 0 0 0 0 0 possible causes and resolution cause: missing isoagglutinins resolution: 1. repeat reverse typing with 4 drops of serum 2. incubate at room temp 30 mins 3. run auto control and sreening cells
anti-A, Anti B, A1, A2, B cells, O cells,auto 4+ 0 1+ 0 4+ 0 0 possible cause: A2 with anti-A1 resolution: 1. type with anti-A1 2. test serum with additional A1 and A2 cells
anti-A, Anti B, A1, A2, B cells, O cells,auto 4+ 4+ 2+ 2+ 2+ 2+ 2+ possible cause: rouleaus resolution: 1. use washed RBC's cells in saline for forward grouping 2. Perform saline replacement tech in reverse grouping
anti-A, Anti B, A1, A2, B cells, O cells,auto 3+ 4+ 1+ 0 0 0 0 possible cause: A2 B with anti-A1 resolution: 1. type cells with anti-A1. 2 test serum with additional A1 and A2 cells.
anti-A, Anti B, A1, A2, B cells, O cells,auto 4+ 2+ 0 0 0 0 0 possible cause: acquired B antigen. resolution:1. check medical history for GI problen or septicemia. 2. type with human anti-B acidifed to pH 6.0 or monoclonal anti-B.
anti-A, Anti B, A1, A2, B cells, O cells,auto 4+ 4+ 2+ 0 0 2+ 0 possible cause: AB with cold alloantibody. resolution: 1. Perform antibody panel. 2. Repeat forward grouping with B cells lacking the corresponding antigen.
ABO TYPING ABO TYPING
ANTI-A, ANTI-B, A CELLS, B CELLS 0 0 + + GROUP O
ANTI-A, ANTI-B, A CELLS, B CELLS + 0 0 + GROUP A
ANTI-A, ANTI-B, A CELLS, B CELLS 0 + + 0 GROUP B
ANTI-A, ANTI-B, A CELLS, B CELLS + + 0 0 GROUP AB
ABO SYSTEM ABO SYSTEM
ANTIGENS ON RBC ANTIBODY IN SERUM neither Anti-A, Anti-B GROUP O
ANTIGENS ON RBC ANTIBODY IN SERUM A Anti-B GROUP A
ANTIGENS ON RBC ANTIBODY IN SERUM B Anti-A GROUP B
ANTIGENS ON RBC ANTIBODY IN SERUM A and B Neither anti-A nor anti-B GROUP A and B
ABO GENOTYPES/PHENOTYPES ABO GENOTYPES/PHENOTYPES
PHENOTYPE A AA, AO
PHENOTYPE B BB, BO
PHENOTYPE AB AB
PHENOTYPE O OO
DIRECT (DAT) vs. INDIRECT (IAT) DIRECT (DAT) vs. INDIRECT (IAT)
in-vivo sensitization of RBC's by IgG antibody is dettected by DAT or IAT DAT
In vitro sensitization of RBC's by IgG antibody is detected by DAT or IAT IAT
What specimen is used for DAT EDTA
does DAT require incubation NO
What specimen is used for IAT serum, plasma or RBC's
DAT detects what (4) 1. Hemolytic disease of the newborn 2. transfusion reactions 3. autoimmune hemolytic anemia 4.drug-induced hemolytic anemia
IAT detects what (4) 1. antibody screen 2. crossmatch 3. RBC phenotyping 4. weak D testing (Du)
DAT false positives: (6) 1. complement binding in vitro if RBC's taken from a red top and AHG used. 2. septicemia 3. contamination of specimen 4. Wharton's jelly in cord blood 5. overreading 6. overcentrifugation
IAT false positives: (2) 1. cells with positve DAT 2. Overcentrifugation
DAT false negatives: (6) 1. interrupting testing 2. contamination, improper storage, outdated AHG. 3. Failure to add AHG 4. Neutralization of AHG 5. Dilution of AHG by residual saline 6. over/undercentrifugation
IAT false negatives: (same as DAT) plus: over/under incubation
Created by: nizhoni