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clls-203 lab

lab analysis final

QuestionAnswer
electrical conductivity that physically detects a clot mechanical endpoint
Used to measure light scattered at a 90 degree angle by particles in solution nephelometry
decrease in light flow detects clot formation utilizes what method photo-optical endpoint
process of generating photons of light via a chemical reaction chemilluminescence
methodology used in urinalysis dipstick, micro biochemical & routine chemistry testing spectrophotometry
separating complexes based on mobile phase and stationary phase chromatography
in electrophoresis, particles are separated on the basis of net charge, size and shape
optimal membrane choice for ISE measurement of pH glass
mechanical endpoint coagulation studies
spectrophotometry urinalysis biochemical testing
flow cytometry automated cell differential
electrical impedence complete blood count
flame photometry concentrations of Na+, K+ and Li+
measures analyte separated by chromotography Mass spectrophotometry
measure electromagnetic radiation atomic absorption
measures emitted light generate by a chemical chemilluminescensce
a holmium oxide filter is used to determine what wavelength accuracy of a spectrophotometer
coulter principle electrical impedence
photometry measures light intensity without regard to wave length
spectrophotometry measures light intensity with regard to wavelength
nephlometry measures scattered light at a 90 degree angle
Beer's law in spectrophotometry relation of absorbance and transmittance to concentration
potentiometry electrochemical cell = 2 half cells
labeled and unlabeled analyte introduced at same time and may attach to binding sites on the antibody competitive immunoassay
type of immunoassay that does not require separation of bound and free factions homogenous
value which occurs with the greatest frequency mode
calculated average of values mean
value at the center/midpoint fo the observation median
closeness of the agreement between the measured value of an analyte to its true value accuracy
bias in 1 direction that displaced the mean shift
gradual increase or decrease in QC values trend
FPIA competitive
MEIA sandwich
EMIT competitive
obtain the same result time after time precision
attraction of antibody/antigen pair, likelihood of binding affinity
after binding likelihood of separation avidity
separation techniques adsorption, precipitation, solid phase
adsorption particles are added that trap antigen, labeled or unlabeled
precipitation environment is altered to affect solubility of protein by adding a substance
solid phase use to immobilize reagant antibody or antigen
quality assurance every aspect of the lab
quality control analytical piece
maximum # of specimen that can be analyzed in a given time throughput
random access testing any test can be run on any specimen
sequential testing series of tests are run on a sample
continuous flow analyzer all samples flow through a common reaction vessel
centrifugal analyzer reagent and sample in a cuvette on a rotor. Reaction occures by spinning
discrete sample analyzer each sample travels in its own reaction vessel
substance that is dissolved in a liquid solute
to make a critical dilution and measurement one should use a volumetric pipette
A pipette with TC needs to be blown out
in the SI system temp is measured on what scale Kelvin
This type of microscopy is used to see unstained elements such as platelets phase contrast microscopy
temp at which water boils in Fahrenheit 212 degrees
type of plastic used in a laboratory polystyrene and polycarbonate
types of glass used in a laboratory pyrex and borosilicate
agency that oversees chemical purity IUPAC
Created by: pamela18