Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Subcutaneous Mycoses
Mod 2 Lec 2
| Question | Answer |
|---|---|
| Subcutaneous mycoses 4 major infections | Mycetoma, Chromoblastomycosis, Phaeohyphomycosis, Sporotrichosis |
| Common to all subcutaneous mycoses | Lesion develops at site of inoculation (localized); soil saprophytes that are moderately slow growers; most are dematiaceous fungi |
| Types of conidiation seen in dematiaceous fungi | Cladosporium type, Phialophora type, Rhinocladiella type |
| Cladosporium type conidiation | Resembles a tree, conidiophore is trunk and branched chains of conidia form branches |
| Phialophora type conidiation | Short conidiophores + phialide, vase shaped, conidia extrude from phialide and then cluster |
| Rhinocladiella type conidiation | Stalked conidiophores that become knobby as conidia are produced, conidia produced sequentially until a cladosporium type conidiation is reached |
| Media used for identification of subcutaneous fungi | SAB with and without antibiotics; PDA for subculture |
| Mycetoma | Chronic granulomatous disease of feet; enlarges nodules, sinus drainage, bone destruction; granules in exudate; no lymphatic system involvement |
| Two types of granules in mycetoma | Actinomycotic(narrow hyphae) and Eumycotic(wide hyphae, ending in chlamydoconidia) |
| Chromoblastomycosis | Localized disease of skin and subcutaneous tissue; wart-like (verrucoid) lesions on feet, legs, hands, buttocks; soil saphrophytes introduced by trauma; dematiacious |
| Chromoblastomycosis - what is seen microscopically | Single celled or clusters of single cells, dark pigment |
| Chromoblastomycosis - setting up for culture | SAB at room temp, hold for 6 weeks |
| What are you looking for in chromoblastomycosis | 3 types of conidiation: cladosporium type, rhionocladiella type, phialophora type |
| Phaeohyphomycosis | Infection of subcutaneous tissue, caused by misc. dematiaceous fungi, introduced through trauma |
| Phaeohyphomycosis - KOH shows | Pigmented hyphae |
| Cause of chromoblastomycosis | Cladophialophora carrioinii |
| Cladophialophora carrioinii macro | Slow grower - up to 30 days; grey-green to black surface and reverse, cottony |
| Cladophialophora carrioinii micro | Pigmented, septate hyphae; cladosporium type conidiation |
| Causes chromoblastomycosis and phaeohyphomycosis | Fonsecaea pedrosoi and Phialophora verrucosa |
| Fonsecaea pedrosoi macro | Grey-green to black, cottony; slow grower, w/in 21 days |
| Fonsecaea pedrosoi micro | Pigmented, spetate hyphae; all 3 types of conidiation: phialophora, cladosporium, rhinocladiella |
| Phialophora verrucosa macro | Olive-green to black, velvety |
| Phialophora verrucosa micro | Pigmented, septate hyphae; only phialophora type conidiation |
| Major etiologic agent of mycetoma in US and Europe | Pseudallescheria boydii |
| Pseudallescheria boydii macro | Grows rapidly; white to brownish-grey, fluffy |
| Pseudallescheria boydii micro | Hyaline, septate hyphae; single anelloconidia produced on an anellophore (conidiophore) |
| How is Pseudallescheria boydii different from other subcutaneous mycoses | Grows rapidly, hyaline, has a sexual form |
| Cause of Mycetoma and phaehyphomycosis by minor trauma and contaminated fomites | Exophiala jeanselmei |
| Exophiala jeanselmei macro | Young cultures: appear as black yeast; Mature cultures: velvety colonies |
| Exophiala jeanselmei micro | Stick-like conidiophores with clustered conidia |
| Wangiella dermatitidis causes | phaeohyphomycosis |
| Wangiella dermatitidis macro | Initially resembles black yeast; > 10days, olive-grey to black, velvety or glabrous; grows better at 40-42C |
| Wangiella dermatitidis micro | Pigmented, septate hyphae; conidiophores are indistinguishable from vegetative hyphae except conidia are clustered at ends |
| Acremonium spp cause | Etiologic agent of mycetomas, corneal infections, and nail infections |
| Madurella grisea macro | Folded in center with radial grooves, dark grey to olive-brown, reverse dark |
| Madurella grisea micro | Dark, thick hyphae; other structures may be absent |
| Madurella mycetomatis macro | Slow growing, fluffy white, grows best at 37C, reverse dark |
| Madurella mycetomatis micro | Dark, thick hyphae, other structures may be absent, some have tiny flask-shaped phialides |
Created by:
blackicecreamcat
Popular Laboratory Science sets