STI Labs Word Scramble
|
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Question | Answer |
Wet prep swab | cotton swab; rotate over vaginal wall/inflammation; avoid cervical mucus/blood; in 1 mL saline |
Wet prep spec to lab within: | 20 min (in 1 mL saline) |
Cervical testing | no bathe 24 hrs; unlubricated speculum; remove cerv mucus w/cotton swab; sterile swab into canal 15-30 sec; plate or media |
Gono cx plates | TM, choc, or Jembec (CO2 reservoir); swab in Z pattern & cross streak; 36C (don’t refrigerate) |
Gram stain | urethra: Ca alginate 1-2 cm x 3-5 sec (females more shallow); cervic: cotton swab, usu not done (gold std for clue cells) |
Oropharyngeal NG cx | swab posterior pharynx & tonsillar crypts; include areas of inflammation or exudate |
Urethral NG cx | Collect ≥1 hr post urination; ideal: prior to first morning micturition; swab anterior urethra |
HSV cx collection | Unroof vesicle lesion (18 ga needle), abrade w/cotton swab; crusted: remove crust w/moist gauze, scrape w/cotton swab; transport medium, refrigerate if delay |
HSV serology | less sensitive; helpful if IgM or high IgG |
4-fold rise in HSV titer indicates: | acute initial HSV infxn |
Darkfield for syphilis | pos = diagnostic; not definitive if neg |
Syphilis: nontreponemal tests | RPR more sensitive than VDRL; VDRL positive 2 weeks after inoculation, positive thru secondary and often in tertiary |
All syphilis tests 100% sensitive in stage: | secondary |
Syphilis: treponemal test | FTA; more specific, pos 4-6 weeks post inoculation; FTA-Abs (sandwich): more sensitive = definitive test; MHA-TP |
NG tests | gram stain, cx (specify suspicion), NAAT (DNA by PCR) |
Chancroid: cofactor in contracting: | HIV |
Chancroid tests | cx (specify suspicion), DNA PCR, no serology |
Chlamydia tests | Lipase chain reaction (LCR) most sensitive/specific. Direct fluoro Ab. NAAT (DNA by PCR). Cx & serology outdated |
HIV requirement: | Patient must be informed of HIV testing; informed consent/ counseling no longer required nationally |
HIV Detuned testing | both sensitive & low-sensitivity tests; if only 1 is pos = recent infxn |
HIV p24 test | free antigen or bound antigen/Ab complexes; detectable 2-6 weeks post infection |
HIV p24 to dx: | viral Rx, neonatal infxn, detect HIV before seroconversion, dz progression |
HIV viral load | reflects Rx response better than CD4; detects transplacental transmission |
LGV test | complement fixation |
GN bipolar rods encapsulated in mononuclear lymphs = | Granuloma inguinale (donovan bodies) |
Gold standard for HSV testing | Viral culture. |
In HSV, Tzanck smear reveals: | multinucleated giant cells |
CSF for neurosyphilis | Unreliable to exclude Dx of syphilis, but useful to document resolution after tx |
Created by:
Abarnard
Popular Laboratory Science sets