Enzyme Kinetics Word Scramble
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| Question | Answer |
| What is the Michaelis Menten equation? | v=Vmax [S] / [S] + Km |
| Define Vmax. | The rate of the reaction at saturation substrate concentrations for a given amount of enzyme. |
| The rate of the reaction at saturation substrate concentrations for a given amount of enzyme defines what concept? | Vmax |
| Define Km. | The substrate concentration that results in 50% Vmax. |
| The substrate concentration that results in 50% Vmax defines what? | Km |
| An inhibitor that binds to the active site and competes with the substrate for its occupation is called a _________ inhibitor. | Competitive inhibitor |
| An inhibitor that binds to, and takes an enzyme out of commission is called a ____________ inhibitor. | Noncompetitive inhibitor |
| Competitive inhibitors ____________ (increase / decrease) the apparent Km. | Increase |
| In a Lineweaver Burke plot, a competitive inhibitor will have _______________ (same / different) point on the x axis. | Different |
| In a Lineweaver Burke plot, a noncompetitive inhibitor will have _______________ (same / different) point on the x axis. | Same |
| In a Lineweaver Burke plot, a competitive inhibitor will have _______________ (same / different) point on the y axis. | Same |
| In a Lineweaver Burke plot, a noncompetitive inhibitor will have _______________ (same / different) point on the y axis. | Different |
| This type of inhibitor binds to the active site and is acted upon by the enzyme just as if it was a normal substrate. The enzyme uses its own catalytic machinery to inactivate itself. | Suicide inhibitor |
| Fluorouracil is what type of inhibitor? | Suicide inhibitor |
| The Y axis on the Lineweaver Burke plot indicates ___________. | 1/Vmax |
| The X axis on the Lineweaver Burke plot indicates ____________. | -1/Km |
| The Y axis on the Eadie-Hofstee plot indicates _________. | Vmax |
| The X axis on the Eadie-Hofstee plot indicates _________. | Vmax/Km |
| The slope of the Eadie-Hofstee plot indicates __________. | -Km |
| True or False: Both competitive and noncompetitive inhibition is reversible. | True |
| True or False: Both competitive and noncompetitive inhibition is irreversible. | False |
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