Pos DAT Word Scramble
|
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Question | Answer |
Reasons for positive DAT | autoantibody, alloantibody reacting w/donor cells, HDN, drug antibodies coating RBCs, complement on RBC, nonspecific immunoglobulins |
Secondary causes of WAIHA | SLE, lymphoproliferative disease |
WAIHA test results | DAT: positive, Serum:reacts with all cells including pts, Eluate: reacts with all cells, XM: incompatible |
Treatment of WAIHA | steroids, rituximab, IVIG, cytotoxic drugs, splenectomy |
___________ adsorption if recently transfused | Allogeneic |
___________ adsorption if not recently transfused | Auto |
To XM for WAIHA, if complex specificity use | 1. least incompatible 2. ABO/Rh compatible 3. In vivo XM |
in CAS, what is the only protein on the RBC? | C' |
Primary CAS | Chronic. Monoclonal prot w/ kappa light chains. Older pop with lymphoma, CLL, waldenstroms |
What syndrome is associated with primary CAS? | Raynaud's |
Secondary CAS | Acute. seen 2-3 wks post M. pneumonia infection. Polyclonal IgM w/normal kappa and lambda light chains |
CAS lab results | Backtype discrepancy. DAT: C' only, Serum:reacts w/ all cells @ RT, Eluate: no run, XM: incompatible at IS with all |
CAS common specificity | auto anti-I |
PCH | DAT (+) C' only. in children post viral infection (measles, mumps) |
PCH is unique how? | Biphasic IgG. IT fixes C' thru C9 |
Common antibody for PCH? | Auto anti-P |
Test for PCH | Donath Landsteiner test |
Drug dependent | Drugs must be present for detection of antibody |
Drug independent | drug does not have to be present for reaction, it alters the rbc membrane |
NIPA | independent of antibody formation |
Drug dependent 1st type | Antibodies react with drug-treated cells---drug binds to RBC and antibody binds to drug only |
Drug dependent 1st type list of drugs | IV penicillin, piperacillin, 2nd & 3rd generation cephalosporins, isoniazid, chemo |
drug dependent 1st type results | DAT (+) IgG only, Serum: NR, Eluate: NR, XM:compatible |
Resolution for 1st type drug adsorption | incubate SC with drug. Add pt serum and elute---> positive means the drug was causing the problem |
Drug dependent 2nd type | Need only to have drug in serum |
drug dependent 2nd type list | quinidine, phenacetin,acetaminophen, 2nd and 3rd gen cephalosporins |
drug dep. 2nd type results | DAT: C' only, Serum =, Eluate =, XM: compatible |
Resolution for drug sep 2nd type | drug + pt serum then add RBCS = hemolysis or agg |
NIPA | Non specific proteins adsorb to RBC-----it is independent of antibody----serum reacts w/drug coated cells and elute does not |
Drug independent | mimics auto ab---will react with all cells---disappears after drug is stopped but takes several months |
Drug ind. list | A-methyl dopamide, L-Dopa, procainamide, NSAID, 2nd & 3rd ceph, chemo (fluarabine) |
Austoimmune like |
Created by:
aenewsom7
Popular Laboratory Science sets