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microscopes

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Question
Answer
micro pages 28-32   (blank)  
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q. What is the minimum distance that can exist between two points such that the points are observed as separate entities known as?   a. Resolving Power or resolution.  
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q. What is the maximum sesolving power of the best compound Microscope(light microscope) which is sufficient to see most bacterial structures but too low to see viruses?   a. 0.02 um  
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q. What is the maximum magnification of a compound microscope?   a. 1000X  
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q. What type of microscope is used to view living organisms and internal organs can be easily seen with?   a. Phase Contrast Microscope.  
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q.What kind of microscope appears as three diminsional images.   a. Interference microscope.  
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q. What highlights specimens against a dark background and can detect Treponema pallidum( causative agent of syphilis)?   a. Dark field microscope  
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q. What kind is used to examine materials that emit light when light of a different wavelength strikes them?   a. Flourescence Microscopy  
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q. What kind of microscope provides three dimensional pictures of thick structures such as a community of microorganisms and provides detailed sectional views of the interior of an intact cell?   a. Confocal scanning laser microscope.  
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q. What uses a beam of electrons guided by strong magnets, has resolving power of 0.3nm, generally magnifys objects 10,000x-100,000x?   a. Electron Microscope  
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q. what has the highest resolving power?   a. Transmission electron microscope.1,000,000x +  
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q.What kinds of images do Scanning electron microscopes create?   a. 3D surface structures but no internal structures.  
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q. What are the four steps to the Gram Stain?   a. 1. Primary stain 2. Mordent 3. Decolorizer 4. Counter or secondary stain.  
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q. What kind of stains are Gram stains and Acid fast stains?   a. Differential stains.  
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q. Acid fast stains are used to stain members of genus Mycobacterium due to a high lipid concentration of mycolic acid in cell wall, what are the four steps?   a. 1. steamer 2. primary dye 3. decolorizer 4. counter stain(Methylene blue).  
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Created by: tim sopp
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