Core Practicals for Biology
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
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| Describe how the effect of caffeine on heart rate in Daphnia can be investigated practically, and discuss whether there are ethical issues in the use of invertebrates | show 🗑
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| show | 5.Range of caffeine concentrations;
6.Repeats;
7.Water as control / zero caffeine concentration};
8.Control temperature;
9.Similar organisms used;
10.Accurate measurement of DV;
11.Plot results on graph
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| Describe how to investigate the vitamin content of food and drink | show 🗑
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| show | 6.Vitamin C ;
7.Repeated at time intervals
8.Replication ;
9.One controlled variable ;
10.Drawing graph of both sets of results ;
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| Describe how membrane structure can be investigated practically, eg by the effect of alcohol concentration or temperature on membrane permeability | show 🗑
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| show | 6.Indication of what is being used to judge permeability colour of solution, absorbance, transmission ;
7.Permeability can be assessed e.g. use of colorimeter, standard solutions ;
8.Repeats / replicates
9.Plot results onto a graph
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| show | Mesure of vol. of oxygen. volume of oxygen
3.Measure the initial rate of reaction
4.CV e.g. age, part of mussel mass,surface area,Vol of hydrogen peroxide, temperature, concentration, pH
6.Repeats
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| show | 1.Use of root tip;
2.Add acid or acetic alcohol
3.Add stain e.g. Toluidine blue
4.Break open tip with (mounted) needle/eq};
5.Mount in stain /acid / water
6.Squash under coverslip;
7.Warm to intensify staining
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| show | 1.Sample/explants from both(tissues)
2.Aseptic cond.
3.Grow cells in a callus
4.Growth reg.
5.Cells / tissue can differentiate / cells can become whole plants
6.Procedure e.g. agar; leave for a suitable length of time / suitable controlled variable
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| show | 1.Suspending fibres with weights,pulling them with forcemeter
2.Fibres of same diameter,different diameters accounted
3.Fibres of initial length
4.How weights added/forcemeter pulled
5.Measurable endpoint, breaking point stretched to standard length
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| Describe how to determine the tensile strength of plant fibres practically.[2] | show 🗑
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| show | 1.3+ range of ion concentrations
2.Repeats(at each concentration)
3. Uniformity of seedlings (e.g. all
from same parent plant, same age, same
original root length)
4.Solution used should contain other
mineral ions / named mineral ions
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| show | 5. One other variable maintained / kept
constant (e.g. temp, all run for same length
of time, light intensity, volume of mineral
solution)
6.Mechanism of judging root
{growth /eq} (to measure optimum
concentration)
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| Describe how to investigate the antimicrobial properties of plants. | show 🗑
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