Microbiology Media, Tests and Specimen Processing
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show | Nutrient, ferric citrate; Esculin hydrolysis by group D strep turns media brown; sodium desoxycholate inhibits many bacteria
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show | Differential isolation and presumptive ID of group D step and enterococci
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Bile Esculin Azide Agar with Vancomycin components | show 🗑
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Bile Esculin Azide Agar with Vancomycin purpose | show 🗑
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show | Tryptocase soy agar, Brucella agar, or beef heart infusion with 5% sheep blood
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Blood agar purpose | show 🗑
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Bordet-Gengou agar components | show 🗑
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Bordet-Gengou agar purpose | show 🗑
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show | Yeast extract, agar, charcoal, and salts supplemented with l-cysteine HCl, ferric pyrophosphate, ACES buffer, and a-ketoglutarate
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Buffered Charcoal-Yeast Extract (BCYE) agar purpose | show 🗑
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Buffered Charcoal-Yeast Extract (BCYE) agar with Antibiotics components | show 🗑
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show | Enrichment and selection for Legionella spp.
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show | Contains vanco (10 mg/L), trimethoprin (5 mg/L), polymixin B (2500 U/L), amphotericin B (2 mg/L), and cephalothin (15 mg/dL), in a Brucella agar base and sheep blood
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Campy-blood agar purpose | show 🗑
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show | Thioglycollate broth supplemented with increased agar concentration and antibiotics
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show | Selective holding medium for recovery of Campylobacter spp.
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show | Blood-supplemented enrichment medium containing cefoperaozn, vanco, and amphotericin to inhibit GNs, GPs, and yeast (respectively)
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show | Selective medium for isolation of Campylobacter spp.
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show | Peptone base with yeast extract, mannitol, and bile salts; supplemented with cefsulodin, irgasan,and novobiocin; neutral red and chrystal violet indicators
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Cefsulodin-Irgasan-Novobiocin (CIN) agar purpose | show 🗑
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Chocolate agar components | show 🗑
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Chocolate agar purpose | show 🗑
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Columbia colistin-nalidixic acid (CNA) agar components | show 🗑
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show | Selective isolation of GP cocci
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show | Infusion agar base with 5% sheep blood. Reduction of potassium tellurite by Corynebacterium diphtheriae produces black colonies
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show | Isolation of C. diphtheriae
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Eosin methylene blue (EMB) agar (Levine) components | show 🗑
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show | Isolation and differentiation of lactose-fermentating and non-lactose-fermenting enteric bacilli
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show | Peptone base broth with glucose and mannitol. Sodium citrate and sodium desoxycholate act as inhibitory agents
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show | Selective (enrichment) liquid medium for enteric pathogens
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show | Peptone base agar with bile salts, lactose, sucrose, salicin,a nd ferric ammonium citrate.; indicators include bromthymol blue and acid fuchsin
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Hektoen enteric (HE) agar purpose | show 🗑
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MacConkey agar components | show 🗑
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MacConkey agar purpose | show 🗑
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show | A modification of MacConkey agar in which lactose agar has been replaced with d-sobitol as the primary carbohydrate
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show | For the selection and differentiation of E. coli O157:H7 in stool specimens
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show | Peptone base, mannitol, and phenol red indicator; salt concentration of 7.5% inhibits most bacteria
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Mannitol salt agar purpose | show 🗑
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show | Peptone agar base with cornstarch, supplemneted with yeast dialysate, 3% hemoglobin, and horse plasma; antibiotic supplement includes vancomycin (2ug/ml), colistin (5.5 ug/ml), amphotericin B (1.2 ug/ml), and trimethoprim (3 ug/ml)
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New York City (NYC) agar purpose | show 🗑
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Phenylethyl alcohol (PEA) agar components | show 🗑
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Phenylethyl alcohol (PEA) agar purpose | show 🗑
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show | Charcoal agar supplemented with horse blood, cephalexin, and amphotericin B
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Regan Lowe agar purpose | show 🗑
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Salmonella-Shigella (SS) agar components | show 🗑
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Salmonella-Shigella (SS) agar purpose | show 🗑
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show | Peptone and soy protein base agar with yeast extract, dextrose, and buffers; addition of hemin, l-cysteine, and 5% blood enriches for anaerobes
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Schaedler agar purpose | show 🗑
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Selenite broth components | show 🗑
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show | Enrichment of isolation of Salmonella spp.
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Skirrow agar components | show 🗑
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show | Selective for Campylobacter spp.
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show | Contains crystal violet, colistin, and trimethoprim-sulfamethoxazole in 5% sheep blood agar base
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Streptococcal selective agar (SSA) purpose | show 🗑
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Tetrathionate broth components | show 🗑
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Tetrathionate broth purpose | show 🗑
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Thayer-Martin agar components | show 🗑
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show | Selective for N. gonorrheae and N. meningitidis
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Thioglycollate broth components | show 🗑
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Thioglycollate broth purpose | show 🗑
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show | Peptone base agar with yeast extract, bile salts, citrate, sucrose, ferric citrate, and sodium thiosulfate; bromthymol blue acts as indicator
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show | Selective and differential for vibrios
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Todd-Hewitt broth supplemented with antibiotics components | show 🗑
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show | Selection and enrichment for Streptococcus agalactiae in female genital specimens
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show | All-purpose enrichment broth that can support the growth of many fastidious and nonfastidious bacteria
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show | Enrichment broth used for subculturing various bacteria from primary agar plates
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show | Yeast extract agar with lysine, xylose, lactose, sucrose, and ferric ammonium citrate; sodium desoxycholate inhibits GP organisms; phenol red as indicator
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show | Isolation and differentiation of Salmonella and SHigella spp. from other GN enteric bacilli
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Mueller-Hinton agar components | show 🗑
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show | Selective for Haemophilus
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Acetamide Utilization principle | show 🗑
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show | Positive: Pseudomonas aeruginosa
Negative: Stenotrophomonas maltophilia
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show | Determines if an organism uses acetate as its sole source of carbon; breakdown of the sodium acdtate causes the pH of the medium to shift toward the alkaline range, turning the indicator from green to blue
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show | Positive: Escherichia coli
Negative: Shigella flexneri
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show | Determines the effect of a small amount of bacitracin (0.04 U) on an organism; Streptococcus pyogenes is inhibited by the small amount of bacitracin in the disk; other beta-hemolytic streptococci usually are not
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show | Positive: Streptococcus pyogenes
Negative: Streptococcus agalactiae
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show | GPs other than some strep and entero and inhibited by bile; growth in 40% bile and hydrolysis of esculin turns ferric ammonium citrate dark brown (resulting from the combination of esculetin and ferric ions to form a phenolic iron complex)
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show | Positive: Enterococcus faecalis
Negative: Streptococcus mitis
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Bile Solubility principle | show 🗑
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Bile Solubility quality control | show 🗑
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Butyrate Disk principle | show 🗑
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Butyrate Disk quality control | show 🗑
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CAMP test principle | show 🗑
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CAMP test quality control | show 🗑
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Catalase test principle | show 🗑
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Catalase quality control | show 🗑
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Cetrimide test principle | show 🗑
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Cetrimide quality control | show 🗑
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Citrate utilization principle | show 🗑
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show | Positive: Klebsiella pneumoniae
Negative: Escherichia coli
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show | Differentiate Staphylococcus aureus from coagulase-negative staphyococci; Sa produces 2 coags: bound and free; Bound is the "clumping factor" and reacts directly with fibrinogen. Fibrinogen precipitates on the cell, causing clumping
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show | Positive: Staphylococcus aureus
Negative: Staphylococcus epidermidis
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Decarboxylase test (Moeller's Method) principle | show 🗑
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Decarboxylase test quality control | show 🗑
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show | Determins the abiliyt of an organism to hydrolyze DNA; the medium is pale green due to DNA-methyl green complex; if the organism growing hydrolyses DNA, the green color fades, leaving a colorless zone
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show | Determines whether an organism is able to hydrolyze the glycoside esculin
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show | Positive: Klebsiella pneumoniae
Negative: Shigella flexneri
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Created by:
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