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Microbiology Media, Tests and Specimen Processing

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Question
Answer
show Nutrient, ferric citrate; Esculin hydrolysis by group D strep turns media brown; sodium desoxycholate inhibits many bacteria  
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show Differential isolation and presumptive ID of group D step and enterococci  
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Bile Esculin Azide Agar with Vancomycin components   show
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Bile Esculin Azide Agar with Vancomycin purpose   show
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show Tryptocase soy agar, Brucella agar, or beef heart infusion with 5% sheep blood  
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Blood agar purpose   show
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Bordet-Gengou agar components   show
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Bordet-Gengou agar purpose   show
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show Yeast extract, agar, charcoal, and salts supplemented with l-cysteine HCl, ferric pyrophosphate, ACES buffer, and a-ketoglutarate  
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Buffered Charcoal-Yeast Extract (BCYE) agar purpose   show
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Buffered Charcoal-Yeast Extract (BCYE) agar with Antibiotics components   show
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show Enrichment and selection for Legionella spp.  
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show Contains vanco (10 mg/L), trimethoprin (5 mg/L), polymixin B (2500 U/L), amphotericin B (2 mg/L), and cephalothin (15 mg/dL), in a Brucella agar base and sheep blood  
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Campy-blood agar purpose   show
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show Thioglycollate broth supplemented with increased agar concentration and antibiotics  
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show Selective holding medium for recovery of Campylobacter spp.  
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show Blood-supplemented enrichment medium containing cefoperaozn, vanco, and amphotericin to inhibit GNs, GPs, and yeast (respectively)  
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show Selective medium for isolation of Campylobacter spp.  
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show Peptone base with yeast extract, mannitol, and bile salts; supplemented with cefsulodin, irgasan,and novobiocin; neutral red and chrystal violet indicators  
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Cefsulodin-Irgasan-Novobiocin (CIN) agar purpose   show
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Chocolate agar components   show
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Chocolate agar purpose   show
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Columbia colistin-nalidixic acid (CNA) agar components   show
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show Selective isolation of GP cocci  
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show Infusion agar base with 5% sheep blood. Reduction of potassium tellurite by Corynebacterium diphtheriae produces black colonies  
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show Isolation of C. diphtheriae  
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Eosin methylene blue (EMB) agar (Levine) components   show
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show Isolation and differentiation of lactose-fermentating and non-lactose-fermenting enteric bacilli  
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show Peptone base broth with glucose and mannitol. Sodium citrate and sodium desoxycholate act as inhibitory agents  
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show Selective (enrichment) liquid medium for enteric pathogens  
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show Peptone base agar with bile salts, lactose, sucrose, salicin,a nd ferric ammonium citrate.; indicators include bromthymol blue and acid fuchsin  
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Hektoen enteric (HE) agar purpose   show
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MacConkey agar components   show
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MacConkey agar purpose   show
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show A modification of MacConkey agar in which lactose agar has been replaced with d-sobitol as the primary carbohydrate  
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show For the selection and differentiation of E. coli O157:H7 in stool specimens  
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show Peptone base, mannitol, and phenol red indicator; salt concentration of 7.5% inhibits most bacteria  
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Mannitol salt agar purpose   show
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show Peptone agar base with cornstarch, supplemneted with yeast dialysate, 3% hemoglobin, and horse plasma; antibiotic supplement includes vancomycin (2ug/ml), colistin (5.5 ug/ml), amphotericin B (1.2 ug/ml), and trimethoprim (3 ug/ml)  
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New York City (NYC) agar purpose   show
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Phenylethyl alcohol (PEA) agar components   show
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Phenylethyl alcohol (PEA) agar purpose   show
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show Charcoal agar supplemented with horse blood, cephalexin, and amphotericin B  
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Regan Lowe agar purpose   show
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Salmonella-Shigella (SS) agar components   show
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Salmonella-Shigella (SS) agar purpose   show
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show Peptone and soy protein base agar with yeast extract, dextrose, and buffers; addition of hemin, l-cysteine, and 5% blood enriches for anaerobes  
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Schaedler agar purpose   show
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Selenite broth components   show
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show Enrichment of isolation of Salmonella spp.  
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Skirrow agar components   show
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show Selective for Campylobacter spp.  
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show Contains crystal violet, colistin, and trimethoprim-sulfamethoxazole in 5% sheep blood agar base  
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Streptococcal selective agar (SSA) purpose   show
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Tetrathionate broth components   show
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Tetrathionate broth purpose   show
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Thayer-Martin agar components   show
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show Selective for N. gonorrheae and N. meningitidis  
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Thioglycollate broth components   show
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Thioglycollate broth purpose   show
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show Peptone base agar with yeast extract, bile salts, citrate, sucrose, ferric citrate, and sodium thiosulfate; bromthymol blue acts as indicator  
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show Selective and differential for vibrios  
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Todd-Hewitt broth supplemented with antibiotics components   show
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show Selection and enrichment for Streptococcus agalactiae in female genital specimens  
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show All-purpose enrichment broth that can support the growth of many fastidious and nonfastidious bacteria  
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show Enrichment broth used for subculturing various bacteria from primary agar plates  
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show Yeast extract agar with lysine, xylose, lactose, sucrose, and ferric ammonium citrate; sodium desoxycholate inhibits GP organisms; phenol red as indicator  
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show Isolation and differentiation of Salmonella and SHigella spp. from other GN enteric bacilli  
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Mueller-Hinton agar components   show
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show Selective for Haemophilus  
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Acetamide Utilization principle   show
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show Positive: Pseudomonas aeruginosa Negative: Stenotrophomonas maltophilia  
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show Determines if an organism uses acetate as its sole source of carbon; breakdown of the sodium acdtate causes the pH of the medium to shift toward the alkaline range, turning the indicator from green to blue  
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show Positive: Escherichia coli Negative: Shigella flexneri  
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show Determines the effect of a small amount of bacitracin (0.04 U) on an organism; Streptococcus pyogenes is inhibited by the small amount of bacitracin in the disk; other beta-hemolytic streptococci usually are not  
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show Positive: Streptococcus pyogenes Negative: Streptococcus agalactiae  
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show GPs other than some strep and entero and inhibited by bile; growth in 40% bile and hydrolysis of esculin turns ferric ammonium citrate dark brown (resulting from the combination of esculetin and ferric ions to form a phenolic iron complex)  
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show Positive: Enterococcus faecalis Negative: Streptococcus mitis  
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Bile Solubility principle   show
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Bile Solubility quality control   show
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Butyrate Disk principle   show
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Butyrate Disk quality control   show
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CAMP test principle   show
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CAMP test quality control   show
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Catalase test principle   show
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Catalase quality control   show
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Cetrimide test principle   show
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Cetrimide quality control   show
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Citrate utilization principle   show
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show Positive: Klebsiella pneumoniae Negative: Escherichia coli  
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show Differentiate Staphylococcus aureus from coagulase-negative staphyococci; Sa produces 2 coags: bound and free; Bound is the "clumping factor" and reacts directly with fibrinogen. Fibrinogen precipitates on the cell, causing clumping  
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show Positive: Staphylococcus aureus Negative: Staphylococcus epidermidis  
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Decarboxylase test (Moeller's Method) principle   show
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Decarboxylase test quality control   show
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show Determins the abiliyt of an organism to hydrolyze DNA; the medium is pale green due to DNA-methyl green complex; if the organism growing hydrolyses DNA, the green color fades, leaving a colorless zone  
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show Determines whether an organism is able to hydrolyze the glycoside esculin  
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show Positive: Klebsiella pneumoniae Negative: Shigella flexneri  
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