Infectious Disease
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| Indirect immunofluorescent antibody, Weil-Felix reaction, and complement are tests for: | RMSF (Rickettsia rickettsia)
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| Histoplasmosis CBC | Organism in neutrophils & monocytes on Wright-Giemsa PBS; in macrophages on bone marrow smear
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| Histoplasmosis labs | high alk phos, LDH, ferritin; anemia of chronic dz; pancytopenia
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| Tests for Cryptococcus | cryptococcal Ag in CSF/serum; India ink or serology w/latex agglutination; CRAG
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| RMSF labs | CSF: pleocytosis & hypoglycorrhachia. Low platelets & Na, high LFTs & bili. Serum IFA to confirm dx (may not see Ab response for 2 weeks)
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| Clustered bacteria in vacuoles in WBCs; low platelets; leukopenia w/left shift; high LFTs; rising Ab immunofluorescence titer | Ehrlichiosis
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| Histoplasmosis CBC | Organism in neutrophils & monocytes on Wright-Giemsa PBS; in macrophages on bone marrow smear
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| Aspergillosis Dx test: | Bx of lesion
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| Large septate hyphae that branch at a 45 degree angle: | Aspergillus (eg, A fumigatus)
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| Organism grows thick-walled cells with single broad-based bud: | Blastomyces
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| Histoplasmosis labs | Anemia of chronic dz. Pancytopenia, high alk phos, LDH, transferrin in disseminated dz.
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| Sputum cx in Histoplasmosis is usually: | Negative
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| Histoplasmosis labs in immunocompromised patients | BM & blood cx usually positive. AIDS pt with disseminated dz: urine antigen assay 90% sensitive
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| Giardiasis labs | 3 stool specimens each 2 days apart: O&P for cyst & troph. Antigen test cheaper, less sensitive & will not detect other organisms
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| Malaria labs | Thin & thick PBS Q8h x3 days for dx & TOC. Rapid antigen test. High then low WBC, high monocytes, possibly anemia & high LFTs
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| CT or MRI showing ring-enhancing lesions in the brain (corticomedullary junction or basal ganglion) suggests: | Toxoplasmosis (often in immunocompromised pt)
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| Thick white vaginal discharge, hyphae & buds on KOH prep | Candida
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| Test of choice for MAC | Blood cultures (98% sensitivity)
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| Hansen disease may see false positives in these tests | Syphilis, antithyroglobulin Ab, RF
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| Tetanus labs | Moderate leukocytosis, normal CSF, normal Ca
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| Lyme labs | ELISA (serum Ab) with western blot to confirm. Half of pts neg Ab in first few weeks
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| Glanders labs | Serology preferred. 16s rRNA gene sequencing for rapid ID.
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| Glanders on CXR | Military nodules, lung abscesses, lobar, or bronchopneumonia
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| GNR demonstrating bipolar “safety pin” staining with Wright, Giemsa, or Wayson stain = | Yersinia pestis (grows best on blood agar or MacConkey at 35C)
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| Q fever labs | Requires special reference lab testing. TOC is indirect immunofluorescence. Also ELISA & complement fixation. High RF, CRP, ESR +/- LFTs & WBC
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| Smallpox labs | Testing at high-containment (BL-4) facility. Electron microscopy to confirm.
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Created by:
Abarnard
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