Microscopic Examination of Infected Material
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| show | colors all cells and background material a deep blue and for Gram iodine to provide the larger iodine element to replace the smaller chloride in the stain molecule.
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| Gram Stain Procedure *short procedure* | show 🗑
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| Gram Stain Procedure *application* | show 🗑
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| Gram Stain Procedure *results* | show 🗑
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| Gram Stain Procedure *QC* | show 🗑
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| Acid-Fast Stain procedure *principle* | show 🗑
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| show | 1. cover smears with TB auramine-rhodamine T stain for 25 minutes
2. wash
3. move to rack on acid alcohol collection container
4. flood smears with 0.5% acid alcohol for 2 min
5. wash smears
6. move to original staining rack
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| show | 7. Flood smear for 4 minutes in potassium permanganate counter stain
8. wash smears
9. air dry
10. examine with the X16 and X40 objectives of the fluorescent microscope equipped with a filter system comparable to a BG-12 exciter filterand an OG-1 barri
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| show | the direct smear examination is a valuable diagnostic procedure for the detection of mycobacteria in clinical specimens
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| show | Fluorescent stain:
Mycobacteria stain bright orange. Count the number of acid-fastbacilli seen on the smear and report as follows:
1-20 -> number seen
21-80 -> few
81-300 -> moderate
>300 -> numerous
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| Acid-Fast Stain procedure *results Kinyoun and Ziehl-Neelsen stains* | show 🗑
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| Acid-Fast Stain procedure *QC* | show 🗑
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| show | Fluorescent stain
Kinyoun stain
Modified Kinyoun stain (partial acid-fast)
Zeihl-Neelsen stain
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| show | 1. cover smear with carbolfuchsin stain for 5 min
2. wash slide
3. move to staining rack
4. Decolorize w/acid alcohol
5. wash
6. flood with methylene blue counter stain for 1 min
7. wash, drain, air dry
8. examine with oil
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| Acid-Fast Stain procedure *short procedure Modified Kinyoun stain (partial acid-fast)) | show 🗑
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| show | 8. counterstain with methylene blue for 30 sec.
9. rinse and air dry
10. examine with X100 oil immersion objective
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| Acid-Fast Stain procedure *short procedure Ziehl-Neelsen stain* | show 🗑
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| show | crystal violet; iodine; decolorizer; safranin
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| show | Mycobacterium sp.; Nocardia sp.; Actinomyces sp.; Streptomyces sp.; Mycoplasma sp.; L-forms
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