Hematology in Practice - Lab Quiz 1 and Homework
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| What anticoagulant is used to make peripheral blood smears? | EDTA
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| What will stain dark blue (or purple) using a Wright-Giemsa stain? | Cell nucleus
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| Which term means an increase in the variation of cell size? | Anisocytosis
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| In performing a platelet estimate at 1000x magnification, the technologist sees an average of 20 platelets per field. How would you interpret this? | Normal
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| What term means an increase in the average size of erythrocytes? | Macrocytosis
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| What term means a decrease in the average size of erythrocytes? | Microcytosis
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| In performing a platelet estimate at 1000x magnification, the technologist sees an average of 41 platelets per field. How would you interpret this? | Increased
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| When would a tech choose to count 200 WBCs for a differential instead of the normal 100? | If the WBC count is greater than 40.0x10^9/L
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| The instrument reports a total WBC of 133.8 x 10^3/uL but unable to read the % of the various WBCs due to the high WBC count. Perform a manual diff and find 13% segs, 74% lymphs, 8% monos, 2% eos, and 3% basophils. What is the absolute neutrophil count? | 17.4 x 10^3/uL
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| The instrument reports a total WBC of 133.8 x 10^3/uL but unable to read the % of the various WBCs due to the high WBC count. Perform a manual diff and find 13% segs, 74% lymphs, 8% monos, 2% eos, and 3% bas. What is the absolute monocyte count? | 10.7 x 10^3/uL
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| What percentage of red cells are destroyed and replaced daily in a normal adult? | 1%
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| The best definition of anemia is: | A decrease in the oxygen carrying capacity of the blood
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| What lab results would suggest anisocytosis? | RDW= 18.0
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| What lab results would suggest a decreased red blood cell count? | HCT= 30
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| The presence of bluish red blood cells is termed: | polychromasia
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| Renal disease causes a: | normocytic, normochromic anemia
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| Iron deficiency causes a: | microcytic, hypochromic anemia
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| Sideroblastic anemia causes a: | microcytic, hypochromic anemia
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| Manual cell counting methods in automated hematology laboratories are used for all of the following: | very low counts, malfunctioning automated instruments, and counting spinal fluids
does NOT include: routine testing
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| In performing a manual RBC count, the tech counts an average of 8 red blood cells. The specimen was undiluted. What is the RBC count for this specimen? | 400 RBCs/uL
8/5 x 0.004= 8/.02
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| In performing a manual WBC count, the tech counts 155 WBCs on one side and 151 WBCs on the other. The specimen was diluted 1:10. What is the WBC count for this specimen? | 3825 WBCs/uL
((155+151)/2) x 10 / 4 x 0.1
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| In performing a manual WBC count, the tech counts an average of 16 WBCs. The specimen was counted undiluted. What is the WBC count for this specimen? | 40 WBCs/uL
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| Twenty cells are counted in one of the nine large squares of a hemocytometer. The sample is diluted 1:10. How many cells are present per mL? | 2,000
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| On a standard reticulocyte preparation with new methylene blue, there are 100 cells counted with blue-stained granulofilamentous material. The red blood count is 3.22 x 10^9/uL and the hematocrit is 30%. Calculate the reticulocyte count as a percent. | 100 retics/ 1000 RBCs = 1/10= 10%
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| What is a good use of the erythrocyte sedimentation rate (ESR)? | monitor patients with rheumatoid arthritis
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| What can cause a falsely increased erythrocyte sedimentation rate (ESR)? | tube not totally vertical
-this applies to traditional ESRs
--westergren
--wintrobe
--modified wintrobe
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| How do sickle cells or spherocytes interfere with the erythrocyte sedimentation rate (ESR)? | prevent rouleaux formation so falsely decrease
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