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Cholesterol and Bile Acid Synthesis

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Question
Answer
Cholesterol Synthesis Starting materials   Acetyl CoA NADPH ATP  
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HMG-CoA reductase   rate limiting step (target of drug therapy)  
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Products of cholesterol synthesis   Cholesterol NADP+ ADP  
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Location of Cholesterol synthesis   hepatocytes  
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regulated enzyme of cholesterol synthesis   HMG CoA reductase  
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Increasing levels of cholesterol __________ cholesterol synthesis   decreases  
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HMG CoA is active when   dephosphorylated  
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HMG CoA is dephosphorylated with ___________ insulin to glucagon levels.   high  
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HMG CoA is inactivated by ____________ and is then _____________   Glucagon; phosphorylated  
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Most significant factor in regulation of cholesterol biosynthesis   decreasing HMG CoA reductase synthesis  
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Proteolysis of HMG CoA reductase increases as ____________   sterol levels rise  
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Which drug class inhibits this enzyme   statins  
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Extrahepatic cholesterol synthesis   occurs under certain pathological conditions  
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Why is cholesterol esterified   regulation  
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where is cholesterol esterified   on lipoprotiens and in cells  
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How does esterification change regulation   cholesterol ester CANNOT regulate HMG CoA reductatse  
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Two enzymes that esterize cholesterol   LCAT (Lecithin) ACAT (acyl CoA)  
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LCAT   Esterifies cholesterol on lipoprotein that can be transferred from HDL to LDL  
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ACAT   esterifies cholesterol within the cell  
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Extrahepatic cholesterol synthesis   under certain pathological conditions  
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Where are cholesterol esterified/de-esterified in cells?   lipoproteins  
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Esterifcation of cholesterol   changes the properties of cholesterol especially with regard to its control functions  
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LCAT   Lecithin: chelesterol acyl transferase Esteerifies cholesterol on lipoproteins  
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What is transfered to LDL and HDL   Esterfied cholesterol  
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ACAT   esterfies cholesterol within the cell  
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Cholesterol esterase   reveses LCAT and ACAT esterification  
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Bile Salt Synthesis Location   hepatocytes  
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Starting compounds of Bile Salts   Cholesterol, ATP, NADPH  
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4 conjugated bile salts   taurocholic glycocholic taurochenocholic glycohenocholic acids  
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Conjugation of bile salts increases ____________   solubility  
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Solubility enables bile salts to act as   detergents  
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Conjugated bile salts composed of   primary bile salts, cholic acids and chenocholic ascid conjugated to taurine or glycine which are hydrophobic  
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How much bile salts are recycled   > 95%  
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Liver synthesis of bile acid _____ g/day   .2 - .6  
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Amount of bile salts reabsorbed   12-32 g/day  
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bile removed by feces   .2-.6 g/day  
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Why would we want to decrease bile salt reabsorption   drugs which bind to bile acids prevent their reabsorption to increase cholesterol excretion  
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Cholesterol esterase   reverses LCAT and ACAT by de-esterifying cholesterol  
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Bile Salt Exrection   in feces  
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Primary Bile Salts   chenocholic acid and chlic acid  
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Location of bile acid synthesis   hepatocytes  
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starting compounds of bile acid synthesis   cholesterol ATP NADPH  
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Controlled step of bile acid salts   products and substrate!!!!  
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Products of bile acid synthesis   cholic acid and chenocholic acids  
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Conjugated bile salts   primary bile salts bound to hydrophobic taurine or glycine  
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4 conjugated bile salts   taurocholic glycocholic taurochenocholic glyochenocholic acids  
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Bile Salts recycled   > 95% 12-32 g/day  
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Why would we want to pharmachologically bind drugs to bile acids   to prevent reabsorption to increase cholesterol excretion  
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Created by: tjamrose
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