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Tubes used in blood tests

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Color / Additive
Mode of Action / Use
Red Top ADDITIVE None   MODE OF ACTION Blood clots, and the serum is separated by centrifugation USES Chemistries, Immunology and Serology, Blood Bank (Crossmatch)  
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Gold Top ADDITIVE None   MODE OF ACTION Serum separator tube (SST) contains a gel at the bottom to separate blood from serum on centrifugation USES Chemistries, Immunology and Serology  
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Light Green Top ADDITIVE Plasma Separating Tube (PST) with Lithium heparin   MODE OF ACTION Anticoagulates with lithium heparin; Plasma is separated with PST gel at the bottom of the tube USES Chemistries  
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Purple Top ADDITIVE EDTA   MODE OF ACTION Forms calcium salts to remove calcium USES Hematology (CBC) and Blood Bank (Crossmatch); requires full draw - invert 8 times to prevent clotting and platelet clumping  
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Light Blue Top ADDITIVE Sodium citrate   Light Blue Top ADDITIVE Sodium citrate MODE OF ACTION Forms calcium salts to remove calcium USES Coagulation tests (protime and prothrombin time), full draw required  
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Green Top ADDITIVE Sodium heparin or lithium heparin MODE OF ACTION Inactivates thrombin and thromboplastin USES For lithium level, use sodium heparin For ammonia level, use sodium or lithium heparin   Green Top ADDITIVE Sodium heparin or lithium heparin MODE OF ACTION Inactivates thrombin and thromboplastin USES For lithium level, use sodium heparin For ammonia level, use sodium or lithium heparin  
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Dark Blue Top ADDITIVE EDTA- MODE OF ACTION Tube is designed to contain no contaminating metals USES Trace element testing (zinc, copper, lead, mercury) and toxicology   MODE OF ACTION Tube is designed to contain no contaminating metals USES Trace element testing (zinc, copper, lead, mercury) and toxicology  
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Light Gray Top ADDITIVE Sodium fluoride and potassium oxalate   MODE OF ACTION Antiglycolytic agent preserves glucose up to 5 days USES Glucoses, requires full draw (may cause hemolysis if short draw)  
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Yellow Top ADDITIVE ACD (acid-citrate-dextrose)   MODE OF ACTION Complement inactivation USES HLA tissue typing, paternity testing, DNA studies  
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Yellow - Black Top ADDITIVE Broth mixture   MODE OF ACTION Preserves viability of microorganisms USES Microbiology - aerobes, anaerobes, fungi  
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Black Top ADDITIVE Sodium citrate (buffered)   MODE OF ACTION Forms calcium salts to remove calcium USES Westergren Sedimentation Rate; requires full draw  
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Orange Top ADDITIVE Thrombin   MODE OF ACTION Quickly clots blood USES STAT serum chemistries  
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Light Brown Top ADDITIVE Sodium heparin   MODE OF ACTION Inactivates thrombin and thromboplastin; contains virtually no lead USES Serum lead determination  
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Pink Top ADDITIVE Potassium EDTA   MODE OF ACTION Forms calcium salts USES Immunohematology  
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White Top ADDITIVE Potassium EDTA   MODE OF ACTION Forms calcium salts USES Molecular/PCR and bDNA testing  
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