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Micro, lab 24

Micro 204, Catalase and oxidase produciton lab 24

X is a respiratory enzyme found in most aerobic organisms. It is tested for by the addition of the chemical reagent X. Catalase, hydrogen peroxide
The catalase test is especially valuable in distinguishing between the gram (+) cocci X, and X, and in distinguishing between the gram (+) long rods X, and X. Staphylococcus (catalase +) Streptococcus (catalase - ) Bacillus (catalase +) clostridium (catalase -)
X organisms do not produce catalase and are poisoned by the accumulation of hydrogen peroxide. Anaerobic
An obligate anaerobe would be expected to give a X test for oxidase. negative
A X color appears in a positive oxidase test. deep purple
The chemical X is added to test for the production of oxidase. tetramethyl-para-phenylenediamine
A positive oxidase test depends upon the presence or absence of X in the cell. cytochromes c
Oxidase production has no value in classification. T or F False
X reagent tests to see if an organism is catalase positive: hydrogen peroxide
Positive catalase test means that: catalase was produced by the organism
X reagent tests to see if an organism is oxidase positive Kovac's oxidase reagent
Positive oxidase test means that: an aerobic organism with c type cytochrome, can oxidize certain amines
Created by: kmorris8



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