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MCBL Electrophoresis

Electrophoresis and Factors

QuestionAnswer
DNA molecular weight Longer the fragments slower it moves, shorter the fragments faster it move
Voltage slower you run the gel better the DNA fragments
Agarose larger the pore size faster the DNA moves; HIGHER the concentration SMALLER the pores; nicer separation (slower run)
Buffer effect migration
Visualization loading gel (movement) stain (DNA fragments)
Created by: aduchini
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