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Microtechniques

fixatives, additives, clearants, dehydrants

QuestionAnswer
Celloidin CNS Less Shrinkage, Less Hardening Hazzardous
Carbowax H2O soluble retains lipids won't infiltrate tissue
Benzene Fast penetration toxic
toulene clears faster than etOH flammable volitile
xylene hydrocarbon miscible with paraffin increase hardness flammable
tetrahydrofuran universal solvent rapid penetration less shrinkage miscible w/alcs. H2O. paraffin, acetone, chloroform toxic dyes insoluble
dioxene rapid less shrinkage less hardening toxic
ethanol dehydrant graded alcohols(70%-100%) fast penetration increased shrinkage increased hardening (over time)
methanol colorless Blood Smears Poisonous
isopropyl alcohol less money not federally funded fast penetration less shrinkage less hardening eosin INSOLUBLE
acetone fats penetration less money<etOH SUPER SHRINKAGE
rubber/bees wax increased stickiness decreased brittleness *Of block, additives to paraffin
Bouin's fixative Acetic acid, Picric acid, Formalin less shrinkage increased Trichromes LYSES RBC
Hollande's fixative Bouin's revised added copper sulfate to Stabilize RBC used in GI tract
Gendre's Bouin's revised: alcoholic preserves Glycogen well
B-5 fixative Mercury choloride, Na sulfate, formalin. NUCLEAR DETAIL will harden after 8hrs.
mercury chloride NUCLEAR DETAIL decreased penetration increased Shrinkage mercury pigment DEADLY
How do we remove mercury pigment? With Iodine followed by Na thiosulfate
Zinc Salts Replace mercury preserves antigenicity hazzardous
k dichromate MYLIN SHEATH fixes MITOCHONDRIA dissolves DNA hazz.
Picric Acid NUCLEAR DETAIL preserves GLYCOGEN (like Gendre's) Decreased Pentration INcreased Shrinkage BOOM
Glyoxal Less toxic Little artifact LYSES RBC dissolves eosinophils
Acetic Acid Fast Penetration nucleoproteins preserved INCREASED SWELLING LYSES RBC
Osmium tetroxide preserves LIPIDS Retains tissue morphology decreased penetration
gluteraldehyde retains good morphology decreased penetration INcreased Hardness Fixes as it penetrates, slowly.
Created by: 681205872
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