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Micro Lec Chap3
(test 2/21)
Question | Answer |
---|---|
Simple Microscope | 1 lens |
Compound Microscope | 2 lenses |
Total Magnification | objective lens x ocular lens |
Condenser | bends light to focus beams of light on object |
Iris Diaphram | allows amount of light |
Resolution | ability of the lenses to distinguish 2 points; shorte wavelengths = greater resolution |
Contrast | staining bacteria or background; dark purple vs. lilac; bright vs. dark |
Refractive Index | light bending ability of medium; immersion oil keeps light from bending |
Brightfield Microscopy | DARK objects visible on LIGHT background |
Darkfield Microscopy | LIGHT object on DARK background |
Phase Contrast | accentuates diffraction that passes through object |
Differential Interference Contrast Microsopy | DIC |
Fluorescence | uses UV light; absorbs UV light & emits visible light; fluorescent dyes |
Electron Microscopy | uses electrons instead of lights; higher magnification |
PREP | 1. Smear, 2. Fix to slide, 3. Stain |