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Micro Lec Chap3

(test 2/21)

QuestionAnswer
Simple Microscope 1 lens
Compound Microscope 2 lenses
Total Magnification objective lens x ocular lens
Condenser bends light to focus beams of light on object
Iris Diaphram allows amount of light
Resolution ability of the lenses to distinguish 2 points; shorte wavelengths = greater resolution
Contrast staining bacteria or background; dark purple vs. lilac; bright vs. dark
Refractive Index light bending ability of medium; immersion oil keeps light from bending
Brightfield Microscopy DARK objects visible on LIGHT background
Darkfield Microscopy LIGHT object on DARK background
Phase Contrast accentuates diffraction that passes through object
Differential Interference Contrast Microsopy DIC
Fluorescence uses UV light; absorbs UV light & emits visible light; fluorescent dyes
Electron Microscopy uses electrons instead of lights; higher magnification
PREP 1. Smear, 2. Fix to slide, 3. Stain
Created by: kwright0005
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