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Impact of Genomics

QuestionAnswer
Cloning Amplification of a a recombinant DNA vector within a host
Restriction Enzymes isolated from prokaryotes that recognize and cleave specific target sequence in DNA
Palindromic Property of most restriction sites; both strands have the same sequence in antiparallel orientation
Specificity Property correlated with restriction site length; as length increases the number of cuts made in sample DNA decreases
Phosphatase Enzyme used to prevent vector (or donor DNA) re-ligation
Klenow Polymerase Enzyme used to generate blunt DNA end from cohesive ends
Gel Electrophoresis Procedure that uses current to move molecules though a porous matrix
Hybridization Screen Procedure used to identify gene of interest in a DNA library
Southern Blot DNA is separated with electrophoresis, blotted, probed
Northern Blot RNA is separated with electrophoresis, blotted, probed
Western Blot Protein is separated with electrophoresis, blotted, probed
Northern Blot used to determine in which tissue a gene is expressed or to detect splice variants
Southern Blot used to determine which positive samples contain the desired segment of a gene of interest
Genomics Study of genome function, regulation, and evolution at a systemic level
Bioinformatics Study of the information content of genomes
Synteny Regions of chromosomes conserved between species
Infection Use of a lytic phage as the host for a recombinant vector
Transduction Use of an inactivated phage to insert a recombinant vector into a bacterial host
Transformation Use of heat shock to insert a recombinant vector into a bacterial host
Adaptor In the production of cDNA, restriction site blunt-ligated to the 5' end of the (former) mRNA rendered in DNA
cDNA dsDNA copy of processed mRNA
PCR Use of thermo-cycles to amplify a segment of DNA; requires reverse complement primers
RFLP PCR-based assays of variable number tandem repeats (micro-satellites) used in DNA fingerprinting
Shotgun Sequencing Genome is digested, cloned, sequenced, then overlapped to produce contigs of progressive size
Created by: allengautier
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