turns on alcohol oxidase promoter which is attached to HRP protein expression and mat alpha secretion sequence

what does HRP do? (HRP plate assay test)

it is an enzyme that converts substrate TMB to blue product

use oligonucleotide primers that hybridize within HRP gene to amplify a portion of HRP from colony. if you get a PCR product of expected size, it is a stud. if not, it is a dud.

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Microbio Lab 8

HRP Plate Assay and Colony PCR

Question	Answer
methanol	turns on alcohol oxidase promoter which is attached to HRP protein expression and mat alpha secretion sequence
what does HRP do? (HRP plate assay test)	it is an enzyme that converts substrate TMB to blue product
PCR test	use oligonucleotide primers that hybridize within HRP gene to amplify a portion of HRP from colony. if you get a PCR product of expected size, it is a stud. if not, it is a dud.