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Microbio Lab 7

Agarose Gel Electrophoresis and Electroporation

QuestionAnswer
components of DNA loading dye glycerol, zylene cyanol and bromophenol blue. purpose = visualize gel progress and determine rate of travel
glycerol makes the sample heavier so it doesn't flow out of well
xylene cyanol and bromophenol blue dyes for sample
which runs faster? small or large pieces smaller
Gel Star/SYBR Green dye binds to DNA and fluoresces under UV light -visualizes and stains DNA fragments
3 possible forms of undigested plasmid supercoiled, nicked, and concatimerized
Mss1 restriction enzyme that linearizes plasmid DNA
if loud popping is heard during electroporation there is salt and poor transformation and bubbles
electroporation method by which yeast DNA is transformed, cells are shocked into uptake of naked DNA. >90% of cells dies
why are cells allowed to recover? to allow for expression (transcription and translation) of gene
mat alpha signals secretion of HRP out of cell
sorbitol osmotic stabilizer that prevents cell lysis.
Created by: syeonie
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