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Nucleic Acid Methods

QuestionAnswer
What is the main use of pulse field electrophoresis? Separates long chains of DNA like chromosomes
Why do we tag DNA fragments with P32? This allows for creation of DNA probes that can be located easily in specific regions on a chromosome
What is hybridization? When heat is applied to DS DNA and the hydrogen bonds are broken, this denaturing it. When reformed, this is called hybridization
What is the difference between northern and southern blotting? Northern - SS DNA Southern - DS DNA
When does the northern/southern differ from western? Membrane (similar) is placed in a buffered salt solution containing DNA probes
What kind of bacteria cells are DNA plasmids inserted into? competent cells
In short, how is a genomic DNA library made? Genomic dna is cut randomly and all inserts are put into vectors and grown individually in bacteria
What is cDNA made from? mRNA
Where does cDNA reverse transcription begin? Poly-A Tail
What degrades the mRNA strand? RNase H
Created by: neeck
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