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Streptococcus
| Question | Answer |
|---|---|
| what is a hemolysin | enzyme that digests RBC's |
| what are enzymes that digest RBC"s called? | hemolysin |
| how are streptococcus bacteria arranged | in chains |
| three types of hemolysins | alpha, beta, and gamma |
| alpha has what type of hemolysis | incomplete |
| what color-alpha | green-brown |
| beta has what type of hemolysis | complete |
| what color-beta | clear |
| sometimes beta can appear gamma so what test do you perform? | Bile Esculin test |
| how do you perform a Bile Esculin test? | streak and stab using aseptic technique |
| a positive test for Bile Esculin is? | a black color in the tube |
| postive Bile Esculin shows what type of hemolysis? | gamma |
| negative Bile Esculin shows what type of hemolysis? | beta |
| what does the Bile Esculin test for? | if the orgnaism can digest esculin in the presence of bile. |
| what does CAMP stand for? | Christie, Atkin, Munch-Peterson |
| what does the CAMP factor show? | beta hemolysis |
| positive CAMP test shows what type of hemolysis and looks like what | beta hemolysis with an arrow pointing toward staph aureus |
| A disc | Bacitracin |
| P disc | Optochin |
| A disc what type hemolysis | beta |
| P disc what type of hemolysis | alpha |
| how to do a CNA test for hemolysis | cotton swab and lawn |
| why incubate streptococcus in candle jar-CNA plate? | streptococcus organisms are microaerophils |
| name 3 streptococcus bacteria used in lab | streptococcus pyogenes, faecalis, mitis |
| most streptococcus are catalase and oxidase negative or positive | negative |
| streptococcus positive for CAMP | strep agal |
| streptococcus negative for CAMP | strep faecalis |
| streptococcus positive for B.E. | strep faecalis |
| streptococcus negative for B.E. | strep agal |
| streptococcus sensitive for Pdisc | strep pneumoniae |
| streptococcus resistant for P disc | strep mitis |
| streptococcus sensitive for A disc | strep pyogenes |
| streptococcus resistant for A disc | strep agala |
| alpha strep | strep pneumoniae and strep mitis |
| beta strep | strep pyogenes |
| gamm strep | strep faecalis and agal |