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What are hydrolysis reactions?
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What is the substrate and enzyme for gelatin hydrolysis?
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Lab Quiz on Biochem

biochemical tests 1-9

QuestionAnswer
What are hydrolysis reactions? they require hydrolases and water
What is the substrate and enzyme for gelatin hydrolysis? Gelatin and gelatinase
What is the End Product of Gelatin Hydrolysis? Amino Acids
What is the incubation temp and time for Gelatin Hydrolysis? 25 degrees and 48 hrs
What is the indicator for Gelatin Hydrolysis? there is none
What is the substrate and enzyme for Starch Hydrolysis? Starch and Amylase
What is the indicator for Starch Hydrolysis Iodine
What is the end product of Starch Hydrolysis? Glucose
What is the incubation temp and time for Starch Hydrolyis? 37 degrees and 24-48hrs
What is Casein Hydrolysis? Protein Hydrolysis or Proteolysis
What is the substrate and enzyme in Casein hydrolysis? Casein and caseinase
What is the indicator in Casein Hydrolysis? there is none
What is the end product of Casein Hydrolysis ? amino acids
What is the incubation temp and time of Casein Hyrdolysis? 37 degrees and 24-48 hrs
What is the medium used in Lipid/Fat Hydrolysis? Spirit blue agar
What is the substrate and enzyme used in Lipid/Fat Hydrolysis? Triglyceride and Lipase
What is the indicator in Lipid/Fat hydrolysis? Spirit Blue indicator
What is the end product of Lipid/Fat hydrolysis? Fatty acids and glycerol
What is the temp and incubation time of Lipid/Fat Hydrolysis? 37 degrees and 24-48 hrs
What are some non fermenting bacteria? Pseudomonas aeruginosa, Pseudomonas fluorescens, and Alcaligenes faecalis
What parts are used in a Phenol Red Test? Sugar substrate, Phenol Red Indicator, Inverted vial (gas), and Protein
What sugars are used in a Phenol Red Test? glucose, lactose, and sucrose
What is the starting pH of Phenol Red Test? 7.4
What color is a positive Phenol Red Test? yellow and pH less than 7
What color is a negative Phenol Red Test? red and pH more than 7
What is the substrate in Litmus Milk Test? Casein, Lactose, and Litmus Blue indicator
What are the enzymes in Litmus Milk test? Lactase or Beta (B) and Caseinase
What is the indicator in Litmus Milk test? Litmus blue
What are the acidic results of a Litmus Milk test? Acidic pH less than or equal to 4.5 and pink color
What are the basic/alkaline results of a Litmus milk test? pH above or equal to 8.3 and a purple color
End products of Litmus Milk test? Acids, Alcohols, ammonia, soft curd or hard curd formation
Litmus blue indicator turns what color with ammonia production? purple
What enzyme secreted by bacteria result in a hard clot formation? Rennin
what is Peptonization? dissolving of clot by proteolytic enzymes, tube becomes liquid and turns brownish-purple
What is stormy fermentation? Anaerobic gas fermentation in a hard clot at the bottom of tube
Litmus blue reduction? bacteria reduce the litmus blue indicator to a white color
Created by: 1109680222
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