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Micro Exam 2.

Growth curve, controlling oxygen content

QuestionAnswer
Lag Phase Little or no cell division Adapting to a new environment Increase in cell size Metabolically active:synthesis of DNA and enzymes, production of ATP
Log (Exponential) Phase Most rapid cell division Generation time calculated Bacteria most susceptible to antibiotics, radiation, heat, and disinfectants Exponential growth can be maintained by transfer to fresh medium or a chemostat
Stationary Phase Number of deaths = number of new cells formed Metabolic rate declines Period of survival Endospores develop
Death (Decline) Phase Number of cell deaths > number of new cells formed more endospores than vegetative cells Many cells undergo involution (odd shaped abnormal cells; difficult to ID)
Obligate aerobes liquid cultures placed on a shaker
obligate anaerobes anaerobic glove box and chamber; GasPak anaerobic system (CO2 incubator)
Microaerophile candle jar
Created by: ktesta
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