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Microbio-lab exam 1

labs 1-8

QuestionAnswer
Staphylococcus epidermis colony morphology small smooth white colonies
Bacillus subtilis colony morphology medium rough white colonies
Escherichia coli colony morphology large mucoid colonies.
Capsule stain Tests for capsule made of polysacharides. crystal violet stained culture grown on milk agar, destained with copper sulfate. examples Klebsiella pneumoniae Bacillus anthracis, streptoococcus pneumoniae
Endospore stain Malachite green & heat, destain with 95% alcohol, counterstain with safranin. Examples Clostridium botulinium, Bacillus anthracis
Acid-Fast stain Tests for thick mycolic acid layer. Stain with carbol-fuschin and heat, destain with acid-alcohol, counterstain methylene blue. Examples: Mycobacterium tuberculosis, Nocardia, Cryptosporidium.
Caseinase Digests Casein enriched agar, positive will have clear halo, Escherichia coli (+) Bacillus subtilis (-)
Amylase Breaks down starch, pour iodine on starch agar, clear means no starch. Escherichia coli (-), Bacillus subtilis (+)
Lipase Breaks Tween80 (polysorbate80) to oleic acid and polyoxyethylated sorbitol. Positive turns broth pink. Mycobacterium smegmatis (+) Escherichia coli (-)
Gelitinase stab bacteria into gelatin medium, positive is if medium liquefies. Escherichia coli (-) Staphylococcus aureus (+)
Dilution factor formula (volume sample)/(Volume sample+volume blank)
CFU/mL formula CFU/(df*volume used of dilution)
Tetrad four cells in square
Sarcina two tetrads forming cube
Created by: alex01tib
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