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lab practical fcc

lab practial study guide for fcc

Question
blood agar differential media beta alpha gamma hemolysis
hemolysis alpha partial growth beta growth and clearing gamma growth
catalase test + bubbles turns hydrogen proxide into h2o & o2
citrate utilization test blue + green- differental media
EMB agar selective differential gram- lactose ferment rods coliforms
IMViC combine indole mrvp citrate distinguish enterobacteriaceae
MacConkey agar selective differential gram- lactose ferment rods coliforms
mannitol salt selective differenital stapyhlcocci grow s.aureus ferments
mueller-hinton agar kirby baurer method zone of inhibition bigger better
MIO motility indole production ornithine decarboxylation
MRVP MR mixed acid fermentation yellow- red+ VP neutral acetoin butanediol red
OF media (glucose) green- yellow+ w/oil anerobic w/o oil aerobe facultative both
Oxidase test swap blue/purple positive no reaction negative
phenol red fermentation broth yellow acid bubble gas bacis fusica nurtal red orange durham tube ph indicator
phenylalanine deamination test amino acid test
strach agar hydrolysis reation reagent iodine (to see reaction on agar) amylase is excenzyme
urea agar urease red + fusica bacis yellow acidic nh3+co2 pink+ yellow peach-
acid fast +fusica pink blue- mycobacteria
capsule white halo around cell stain cell and background
endospore +organisms bacillus clostridium
flagella motile organisms
gram purple + pink/red -
bile salt inhibit growth of gram+ organisms
COLIFORMS GRAM- LACTOSE FERMENTING ROD FACULTATIVE ANAEROBES(bacullius) ENTERICS USED TO DETECT FECAL CONTAMIATION(emb macconkey)
Colony visible to maked eye on agar plate pure colony
dichotomous key gram- and gram+ flowchart
differenital media differentiate organismis by growth characteristics
Enterobacteriaceae/enterics gram- facultatively anaerobic rod inhibit animal intestines
facultative anaerobe can metabolize aerobically or anaerobically by fermination
fermantation produces acid gas alcohol end products from carbohydrate hydrolysis
obligate aerobe only metabolizes aerobcially of test w/o oil only
resolution what u can see throught a microspoce clearly smaller resolution the better (think camera pixels)
selective media inhibits growth of some organisms favor growth of others
streak plate method for pure colonies...0 1 2 3
unrine culture plate colony counts CFU ul to ml number times 1000
S aureus gram+ purple cluster cocci
E.coli gram- pink/red rods
bacillus spp. gram- pink/red rods
Created by: 100000408129238
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