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MICROBIO EX.29

Triple Sugar Iron Test

QuestionAnswer
What is the purpose of the test? use to differentiate bacteria on the basis of glucose, lactose, sucrose fermentation and sulfur reduction
What causes black precipitate? Fe salt in the medium contact with H2S
Phenol red? if acid? If alkaline? pH indicator. Acid: Yellow, Alkaline: Dark Pink.
E. coli Enterobacteriacae, Gram negative bacilli
A/A? acid produce -> G, L or S fermented to acid.
K/A G fermented to acid and protein break down to alkaline end product.
K/K no sugar fermented. or protein converted to alkaline end product aerobically and anaerobically.
K/- No sugar fermented. protein converted to alkaline product aerobically.
-/- Organism fails to grow.
Blackening along stab line H2S H2S produced.
Bubble,break in the agar caused by gas built up.
Created by: pipi1402
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