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MICROBIO EX.27

Phenylalaline Deamination

QuestionAnswer
What is the purpose of phenylalanine test? To detect the ability of producing phenylalanine deaminase by removing amine group from phenylalanine. (Proteus genus from family Enterobacteriacae)
What does phenylalanine deaminase do? remove NH2, result: phenylpyruvic acid.
Reagent? 12% Ferric chloride.
How to read result? after adding FeCl3? Positive: green->org has phenylalanine deaminase Neg: yellow -> org lacks phenylalanine deaminase.
What is an deaminase? an enzyme, remove NH2, create phenylpyruvic acid.
FeCl3 detect? Phenylalanine deaminase.
The phenylalanine deaminase test can be used to differentiate the genus ______ from other member of the family _______ ? Proteus, Enterobacteriacae.
Created by: pipi1402
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