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MICROBIO EX.26

Decarboxylation of amino acid and various substrate

QuestionAnswer
What is amino acid composed of? Amino group NH2 and Carboxy acid group COOH
what is decarboxylase? An enzyme, remove COOH, result: alkaline amine and CO2
What is lysine? Amino acid, if it is decarboxylated, yield to cadaverine.
Why use lysine? Test for lysine decarboxylase activity.
What is ornithine? Substrate, if is decarboxylated, yield putrescine.
What kind of media is used for decarboxylase test? MIO, Lysine
What is MIO? Motility, Indole, Ornithine
Why we used mineral oil? because it will provide an anaerobic environment for glucose fermentation to acid. Acid will activate decarboxylase enzymes.
Positive result? Purple and turbid throughout -> positive -> ornithine is decarboxylated to putrescine.
Negative result? Purple at the top and yellow below -> negative -> Organism lack of ornithine decarboxylase enzyme. Yellow throughout -> Glucose fermented to acid but organism lack decarboxylase enzyme.
Purpose of the test? To detect the ability of an organism to decarboxylase an amino acid.
Created by: pipi1402
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