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MICROBIO EX.25

Nitrate reduction

QuestionAnswer
Purpose of nitrate reduction? to detect the ability of an org to reduce nitrate to nitrite or some other nitrogenous compound, such as molecular nitrogen N2.
Nitrate reduction is one form of what kind of respiration? anaerobic respiration.
role of NO3? Serve as final electron acceptor when oxygen is unavailble.
This is what most enteric bacteria have this enzyme nitrate reductase.
Nitrate? NO3
Nitrite? NO2
Many NONenteric bacteria contain this enzyme. this enzyme can reduce nitrate beyond the nitrite stage: NO, NH4, N2O, N2
NO? Nitric oxide
NH4 Ammonia
N2O nitrous oxide
N2 nitrogen gas
Reagent for Nitrate? Reagent A: Sulfanilic acid Reagent B: Naphthalamine
Interprete nitrate reduction result? AddA+B:Media turns red: Positive for nitrite -> positive for nitrate reduction AddA+B: media has no color change -> Negative for nitrite -> add Zinc After add zinc: media turn red -> nitrate reduce to nitrite by zinc dust -) Negative 4 nitrate reduction
Continue Add zinc dust: media not change color -> nitrate not reduce to nitrite by Zinc dust -> positive for nitrate reduction beyond the nitrite stage.
Created by: pipi1402
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