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Phlebotomy test tube

Tube ColorAdditiveMode of actionUses
Gold Top None Serum seprator tube (SST) contains a gel at the bottom to separate blood from serum on centrifuge Chemists, Immunology and serology
Light Gray Top Sodium flouoride and potassiun oxalate Antiglycolytic agent preserves glucose up to 5 days Glucoses, requires FULL DRAW (May cause hemolysis if short draw)
Yellow Top ACD (Acid-citrate-dextrose) Complement inactivation HLA tissue typing, paternity testing, DNA studies
Yellow-Black Top Broth mixture Preserves viability of microorganisms Microbiology - aerobes, anaerobes, fungi
Black Top Sodium citrate (buffered) Forms calcium salts to remove calcium Westergen Sedimentation Rate; requires FULL DRAW
Orange Top Thrombin Quickly clots blood STAT serum chemistires
Light Green Top Plasma Separating Tube (PST) with lithium heparin Anticoagulates with lithium heparin; Plasma is separated with PST gel at the bottom of the tube Chemistries
Purple Top EDTA Forms calciun salts to remove calcium Hematology (CBC) and blood bank (Crossmatch); requires FULL DRAW- inver 8 times to prevent clotting and platelet clumping
Light Blue Top Sodium citrate Forms calcium salts to remove calcium Coagulation tests (protime and prothrombin time), FULL DRAW required
Light Brown Top Sodium heparin Inactivates thrombin and thromboplastin; Contains virtually no lead Serum lead determination
Pink Top Potassium EDTA Forms calcium salts Molecular/viral load testing
Green Top Sodium heparin or lithium heparin Inactivates thrombin and thromboplastin For lithium level, use sodium heparin For ammonia level, use sodium or lithium heparin
Red Top None Blood clots, and the serum is separated by centrifugation Chemists, Immunology, and Serology, Blood Bank
Dark Blue Top EDTA Tube is designed to comtain no contaminating metals Trace element testing (Zinc, lead,copper, mercury)and toxicology
Created by: Kimberlym_1992
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