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BIO Enzymes
Protein: Enzymes
| Question | Answer | ||||||
|---|---|---|---|---|---|---|---|
| Enzymes play an important role in (3) | Metabolism | Diagnosis | Therapeutics | ||||
| Enzymes are | Special proteins that act as biological catalysts | ||||||
| Catalyst (define) | Speeds up a chemical reaction | ||||||
| How do enzymes catalyze | increases the rate of reaction by lowering the energy of activation | ||||||
| Are enymes altered or consumed during a reaction? | No | ||||||
| Active site | enzyme molecules special pocket or cleft | ||||||
| Substrate | reactant in biochemical reaction | what catches onto active sites | |||||
| Apoenzyme | without a non-protein moiety | ||||||
| Holoenzyme | active enzyme with its non-protein component | ||||||
| Cofactor | A non protein chemical compound that is loosely bound to an apoenzyme by non-covalent bond | ||||||
| Coenzyme | a type of cofactor | ||||||
| Prosthetic group | a type of cofactor | ||||||
| Examples of Enzymes | Amylase | Protease | Lipase | Lactase | |||
| Enzyme substrate reactions | lock and key model | induced fit model | |||||
| lock and key model | requires substrate and enzymes to fit exactly | ||||||
| induced fit model | active site is flexible, they adjust to maximaze the fit | ||||||
| Three factors that affect enzyme activity | Environemental conditions (temp, pH, substrate concentration) | Cofactors and Coenzymes | Enzyme Inhibitors | ||||
| Environmental Conditions | Temperature? What temp is best? | Extremes are dangerous, 37 degc | Optimum pH? Can some enzymes like acidic or basic conditions? | 7(neutral), yes | |||
| Types of reactions | Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases |