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MLab Sec. 3-1

Microscopy

QuestionAnswer
Condenser lens lens which concentrates the light and makes illumination of the specimen more uniform
Refraction bending of light as it passes through the objective lens.
Total magnification objective lens X ocular lens
Resolution clarity of an image
Limit of resolution/ resolving power An actual measurement of how far apart two points must be for the microscope to view them as being separate.
Numerical aperture a measure of a lens's ability to capture light coming from the specimen and use it to make the image.
Dark-field microscopy a special condenser is used so only the ligt reflected off the specimen enters the objective.
Phase contrast microscopy uses special optical components to exploit subtle differences in the refractive indices of water and cytoplasmic componenets to produce contrast
Fluorescence microscopy uses a fluorescent dye that emits fluorescence when illuminated with ultraviolet light.
Created by: mconduff
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