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Stains

Stain description + steps

Stain TypeDescription & Steps
Gram Stains differentiates bacteria by cell wall structure - Gram +: purple - Gram -: pink/red 1) Primary Stain: crystal violet 2) Moderant: iodine 3)Decolorizer: alcohol 4) Counterstain: safranin
Spore Stain identifies endospores - spores: green - cells: pink 1) Primary stain: malachite green 2) Decolorizer: water 3) Counterstain: safranin
Acid-Fast Stain detects mycolic acid in cell walls 1) Primary stain: carbol fuchsin 2) Decolorizer: water 3) Counterstain: safranin
Negative Stains stains background, not cells - used for capsules - no heat fix 1) Reagent: nigrosin/indian ink
Created by: user-1812645
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