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MMBIO Unit 3

Starting at topic 19

QuestionAnswer
RNA Polymerase The enzyme that carries out RNA transcription. Made up of core enzyme and holoenzyme.
Core enzyme Initially sticks to DNA, 5 subunits, starts transcribing anywhere on certain DNA.
Holoenzyme Core enzyme +Sigma factor (6 subunits). Different sigma factor for different promoter --> only transcribes certain genes.
Basal transcription complex The subunits of RNAP??? DEFINE LATER
Characteristics of RNA synthesis DNA template/sense strand DNA coding/antisense strand ATP, GTP, CTP, UTP w/ 2 hydroxyl groups on ribose (ribonucleotide triphosphates) No primer 5'-->3'
Transcription steps Initiation: bring in proteins to DNA, add first nucleotides Elongation: Building the RNA polymer Termination: stop polymerization, detach proteins
Elements of genes important for RNA transcription -35: TTGACA usually where the promoter is to recruit transcription factors and RNAP upstream of start (16-19 bp) -10: TATAAT pribnow box (5-9 bp) +1: 1st base RNA transcribes
Terminator Transcription stops at this sequence, seq that kicks off RNAP
Created by: lilelise
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