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Quiz 2

All ch. 9 pool

QuestionAnswer
Which of the following is the term for free-living bacteria? Planktonic
Which type of system is used to maintain bacteria in a relatively constant environment, allowing them to stay in the log phase of growth? Chemostat
T or F: MacConkey agar can be used to select for the growth of gram-positive bacteria, making it especially useful to study Enterobacteriaceae. False
T or F: Quorum sensing always occurs between members of the same species. False
Which of the following best describes culture density? The number of cells per unit volume
Capnophiles are bacteria that grow best under which of the following conditions? relatively high CO2 and relatively low oxygen concentrations
What is the goal number of CFUs on a spread plate if a serial dilution is done correctly? 30-300 per plate
The initial cells added to a batch culture are called which of the following? the inoculum
T or F: Fungi can generally thrive in drier environments than can bacteria True
Which of the following is true of using a Petroff-Hausser chamber to do a direct cell count? If the sample was diluted, then the concentration must be corrected accordingly
Which of the following media is commonly used to determine the oxygen requirements of a bacterial species? Thioglycolate medium
Which best describes bacterial growth during the log phase of the growth curve? exponential
Which of the following is the correct order of phases of growth in the growth curve of a batch culture? lag --> log --> stationary --> death
Which best defines the term "persisters?" cells with relatively slow metabolic rates that survive longer than others during the death phase and that can cause chronic infections
T or F: Halotolerant microbes cannot grow without high concentrations of salt False
Which type of microbes thrive in the Great Salt Lake? extreme halophiles
Which of the following is another term for generation time? doubling time
Which of the following is the most common category of bacteria? neutrophiles
Human pathogens are generally which type of microbe? Mesophiles
Which of the following would be the best choice for distinguishing living and dead bacteria in a cell count? primary and secondary fluorescent stains
Food spoilage is often caused by which type of microbe? psychrotrophs
If you grow two species of bacteria on mannitol salt agar to determine which can ferment mannitol, and you know both species can grow on the medium, which type of medium is the agar? Differential
T or F: Generation time in prokaryotes refers to the amount of time required for the doubling of the population through a single round of binary fission. True
In batch culture, how does the log phase of the bacterial growth curve appear? linear of a semi-log scale
A candle jar is often used to grow which type of bacteria? Capnophiles
Which structure separates new daughter cells in binary fission? The division septum
Which of the following best describes FtsZ? The protein that assembles the Z ring, which forms a divisome before division by binary fission
Bacteria most commonly reproduce using which of the following methods? Binary fission
Which of the following best describes why extracellular polymeric substances are important? They are components of a biofilm secreted by the microbes present.
T or F: Estimates of coliforms in water are often made using most probable number techniques True
Which microbial pathogen has an unusually long doubling time? Mycobacterium leprae
Which of the following best describes how a microaerophile would grow in thioglycolate tube culture? A short distance below the top of the medium, but not at the top
Which of the following is an indirect method of measuring numbers of bacterial cells? Turbidity measurement
T or F: The largest component of the dry weight of a biofilm is usually extracellular polymeric substances True
Which of the following is not a reactive oxygen species? Atmospheric oxygen
Which of the following is not an example of an enzyme used to detoxify reactive oxygen species? Permeases
Which of the following is not a common step in preparing a pour plate to make a count of microbial numbers? Bacteria are spread across the agar, using a sterile spreader
Created by: julie.russell
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