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DNA

QuestionAnswer
Why are strawberries particularly effective for DNA extraction? They have more chromosomes per cell than wild strawberries
Which based pairs are purines? Adenine and Guanine
What are the structural differences between purines and pyrimidines? Purines are larger and have double-ring structure; pyrimidines are smaller with a single-ring structure
What three components make up the structure of DNA? Sugar, phosphate, and nitrogen base
Why were proteins detected in the DNA extract? The extraction process did not separate proteins from DNA
Which enzyme is primarily responsible for adding nucleotides during DNA replication? DNA polymerase
What is the nature of DNA replication? Semi-conservative
What is the role of helicase in DNA replication? To unwind the DNA double helix
Why do we keep samples on ice during DNA extraction? To prevent DNA from degrading
What principle does gel electrophoresis rely on? Charge and size
How does the size of a DNA molecule affect its movement in gel electrophoresis? Smaller molecules move faster
What is the first step in pouring a gel for electrophoresis Preparing the agarose solution
How do you read a p20 micropipette? The range is 2-20 microliters
What is the function of ligase in a DNA replication? To join Okazaki fragments together
What is a DNA fingerprint? A pattern of DNA fragments generated by restriction enzymes
Why are restriction enzymes used in DNA fingerprinting? They cut DNA at specific sequences
Besides crime forensics, what is another use of DNA fingerprinting? Paternity testing
What is not a step in the DNA extraction process? Amplifying DNA using PCR
What role do nitrogen bases play in DNA? They encode genetic information
What happens to the DNA during the gel electrophoresis process? It is separated based on size
What is the purpose of adding a loading dye to DNA samples before electrophoresis? To visualize the DNA during loading
What is the main component of the gel used in gel electrophoresis? Agarose
What occurs during the denaturation step of PCR The DNA strands are separated by heat
In gel electrophoresis, what direction do the DNA fragments move? Towards the positive electrode
Created by: ortizal126
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