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Lab practical #2

Photosynthesis, cellular respiration, DNA extraction and GMOs

TermDefinition
Formula for cellular respiration C6H12O6+6O2 -> 6CO2 + ATP +6H2O
Formula for photosynthesis 6CO2 + 6H2O +ATP -> C6H12O6 + 6O2
Independent variable for rate of PS experiment Conc. of CO2/Type of light
Dependent variable of PS experiment Rate of photosynthesis/# of floating disks
Phenol red turns _ when acidic yellow
Phenol red turns _ when neutral red
Phenol red turns _ when basic pink
Steps of DNA extraction Crush food Add water, vortex, centrifuge Add chelex Centrifuge
Why do we crush the food to break apart the cells from each other
Why do we add water, vortex, and centrifuge to break open the cell walls and membranes
What is the main GMO primer CaMV35s
PCR stands for polymerase chain reaction
What enzyme do we use for PCR and why Taq polymerase because unlike DNA polymerase. it comes from a thermophilic bacteria that can withstand high temperatures
Ingredients used in PCR Taq polymerase Nucleotides Primers Template DNA
Steps of PCR Denaturation at 95 deg Annealing at 50-55 deg Extension at 75 deg
Created by: 1763
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