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Biol 1406 Lab Exam 1
Biol 1406 Lab Practical 1 Units 1-7
| Term | Definition |
|---|---|
| Used to measure 5ml or less | Serological pipette |
| Used to measure over 5 mls | Graduated cylinder |
| Used only to store liquids, not to measure | Beaker |
| Accuracy | Estimated values are close to real # |
| Precision | Numbers are close to each other |
| Calculate magnification | Objective lens x ocular lens magnification (always 10x) |
| Oil immersion magnification | 100x |
| Both prokaryotes and eukaryotes have | Plasma membrane and ribosomes |
| 3 domains of life | Bacteria Archaea Eukarya |
| 4 kingdoms of Eukarya | Protists Animalia Plantae Fungi |
| 3 types of bacteria shapes | Bacilli (rod shaped) Cocci (circle) Spirilla (spiral) |
| Gram + bacteria cells | Appear purple under a microscope Thick peptidoglycan cell wall |
| Gram - bacteria cells | Appear pink under a microscope Thin peptidoglycan cell wall |
| C1V1=C2V2 | Find concentration or volume from stock solution |
| Concentration is always in | % |
| If absorbance inc, then concentration _ | also inc |
| If transmittance inc, then concentration _ | dec |
| Formula for starch/maltose | Starch -Amylase> Maltose |
| Starch is the | substrate |
| Amylase is the | enzyme |
| Maltose is the | product |
| Maltose is a _ made of _ | disaccharide/reducing sugar; glucose + glucose |
| The presence of protein in a solution is detected with the _ reagent which turns it _ | Bradford; blue/black |
| In _, the concentration is _ for each tube | Serial dilution; halved |
| The presence of starch is detected by _ which will turn _ | iodine; blue/black |
| The presence of maltose is detected by _ and _, which will turn _ | DNS; heating; brown/amber |
| When exposed to extreme _ and _, _ will lose their _ and _ in a process called _ | temperature; pH; structure; function; protein denaturation |
| Buffers | resist changes in pH |
| Cofactors | Affect/help enzyme activity |
| Osmosis | diffusion of water across a semipermeable membrane |
| Metric conversion scale | K H D O D C M -- micro -- nano -- pico |
| Starch + iodine | blue/black coloring |
| DNS + maltose | brown/amber coloring |
| Maltose + heat | brown |
| Iodine + heat | no color change |
| Why doesn't maltose diffuse out of the membrane | it's too big to go through (polysaccharide) |
| Hypertonic animal cell will | shrivel |
| Hypertonic plant cell will | shrivel |
| Hypotonic animal cell will | explode |
| Hypotonic plant cell will | stay intact |
| Isotonic animal cell will | stay intact |
| Isotonic plant cell will | shrivel |
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