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Biology

Food Tests (Section 2)

QuestionAnswer
What is the test for glucose? Benedict's Test
What is the test for starch? Iodine Solution
What is the test for proteins? Biuret Test
What is the test for lipids? Sudan III Test
Describe the Benedict's Test 1 Transfer 5cm3 of food sample to test tube 2 Set water bath to 75 degrees 3 Add 10 drops of Benedicts solution to tube 4 Leave test tube in water bath for 5 mins 5 Test tube turns from blue to green/yellow in low glucose conc and brick red in high
Describe the test for starch 1. Transfer 5cm3 of food sample into test tube 2. Add few drops of iodine solution and gently shake 3. If starch is present solution will turn from BROWN-ORANGE to BLACK/BLUE-BLACK
Describe the Biuret Test 1. Transfer 2cm3 of food sample to test tube 2. Add 2cm3 of Biuret solution to sample 3. Sample contains proteins if colour goes from blue to pink/purple
Describe the Sudan III Test 1. Transfer 5cm3 of food sample to test tube 2. Add 3 drops of Sudan III solution and gently shake tube 3. If lipids present, solution will stain them and a bright red layer will form at top of mixture
What colour change takes place in the Benedict's Test if high glucose concentrations are present? Blue -------> Brick-Red
What colour change takes place in the Iodine Solution if starch is present? Brown-orange ------> Black/Blue-Black
What colour change takes place in the Biuret Test if protein is present? Blue -----> Pink/Purple
What will appear if lipids are present in the Sudan III Test? A bright red top layer
Created by: JoeMather
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