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Genetics exam
Genetics
Question | Answer |
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Which of the following is is true with regards to bacterial trtansformation? | *free DNA is taken in from the external media only DNA from the same species can be taken in * the DNA gets incorportaed into the host genome |
Which of the following happens during the elongation step of translation? | * anticodon of tRNA binds with complementary mRNA codon *the polypeptide bound to the tRNA at the P-site gets transferred to the amino acid carried by the tRNA in the A-site |
"In the DNA replication process, a ligase protein does what?" | * produces a continuous DNA strand opens the DNA double helix removes tangles in DNA prevents intrastrand H-bonding * seals Okazaki fragments |
Which of the following are features of introns? | GU' bases at the 5' end internal 'A' branch site internal 'AG' binding site |
An organism s genome is discovered to consist of 38% Adenine bases. Using Chargaff's rule, what would be the sum of the proportions of Cytosine and Guanine?" | If A = 38%, T also is at 38%. T+A = 76, so G+C must = 100-76 = 24% |
An autoradiogram was generated by the dideoxy sequencing method. Reading from bottom to top gives you the sequence of the synthesized strand in the 5 to 3 direction. It is 'TGAACGGT'. What would the sequence of template strand be from the 5 to the 3 end? | ACCGTTCA |
Which of the following are nonsense mutations? | * UUA -> UGA UCG -> UAG AAC -> UAC UGC -> UGG |
Which of the following describe the annealing step in a PCR? | * temperature is lowered to around 55C * primers bind to flanking regions of the target DNA |
Which if the following are true with regards to tRNAs? | * the wobble hypothesis refers to to position of the 5' base on the anticodon of the tRNA the wobble hypothesis refers to to position of the 3' base on the codon of the tRNA * some tRNAs can pair with multiple codons of mRNA due to non-standard base pai |
Which of the following were true regarding the Hershey-Chase experiments? | * proved that DNA was the heritable material radioactive sulfur was found outside the bacterial cells * proved that bacteria could transfer DNA between cells |