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BKB Micro Lab Final
BIOL2210 study for Lab final
| Question | Answer |
|---|---|
| What media is used for Butandiol or Acetoin production (VP)? | MR-VP tubes and Vogus-Proskauer reagent A and Barritt's reagent B |
| What media is used for Hydrogen Sulfide production? | SIM Tube |
| What media is used for Indole production? | Tryptone broth with Kovacs reagent |
| What media is used for Starch Hydrolysis? | Starch Agar with Iodine |
| What media is used for Urease production? | Urea Agar |
| What media is used for Citrate Utilization? | Simmons Citrate Agar |
| What media is used for Casein Hydrolysis? | Skim Milk Agar |
| What media is used for Mixed Acid Fermentation (MR)? | MR-VP tubes with Methyl Red |
| What media is used for Glucose Fermentation? | Phenol Red broth with inverted vial |
| What media is used for Nitrate Reduction? | Nitrate broth with Nitrate Reagent A & B |
| What media is used for Catalase production? | Nutrient Agar with H2O2 |
| The appearance of a positive result for Glucose Fermentation without any gas is? | Yellow color without any 'air' in the inverted vial |
| The appearance of a positive result for Citrate Utilization is? | Color change from Green to Blue |
| The appearance of a positive result for Urease production is? | Red color |
| The appearance of a positive result for Indole production is? | Red layer on the top of the medium |
| The appearance of a positive result for Butandiol or Acetoin production is? | Red or Pink color |
| The appearance of a positive result for Hydrogen sulfide production is? | A dark color in the SIM tube |
| The appearance of a positive result for Nitrate reduction is? | A red color appears |
| The appearance of a positive result for Catalase production is? | Foams or Bubbles with the addition of H2O2 |
| The appearance of a positive result for Casein production is? | Clear zone around the streak |
| The appearance of a positive result for Starch Hydrolysis is? | Clear zone around the streak |
| The appearance of a positive result for Mixed Acid Fermentation is? | Red Color |
| What does the Gelatin stab testing for? | The enzyme Gelatinase |
| What does the Fluid Thioglycollate Medium (FTM) testing for? | If it is aerobic or Anaerobic |
| What does the motility Medium testing for? | Flagella |
| Why is blood agar used to test for strep? | If positive, the bacteria form tiny colonies on this medium |
| What is the difference between beta-hemolysis and alpha-hemolysis? | Beta-hemolysis creates clear zones and indicates Strep Throat where alpha-hemolysis creates tan or light green zones and is not strep throat |
| What color zone does a gamma-hemolysis create? | White |
| Blank for printing | Blank for printing |
| Blank for printing | Blank for printing |
| What is the primary stain in gram staining? | Crystal Violet |
| What is the mordant in gram staining? | Iodine |
| What is the decolorizer in gram staining? | Ethanol |
| What is the counterstain in gram staining? | Safranin |
| What is the process for staining | Smear, Heat Fix, Stain |
| The pure culture techniques use what method? | Streak plate method |
| Using the streak plate method on nutrient agar, what color would the coloniesbe for E. coli? | White |
| Using the streak plate method on nutrient agar, what color would the coloniesbe for S. marcescans? | Red |
| Using the streak plate method on nutrient agar, what color would the coloniesbe for M. luteus? | Yellow |
| How do you determine the effectiveness of a disinfectant? | Measuring across the zone of inhibition. The greater the zone, the greater the effectiveness. |
| To use the Enterotube II, what two conditions must exist? | Oxidase Negative and Gram Negative rod |
| Positive results in the Enterotube II are: | Yellow, Purple, Purple, yellow with Black, Yellow, Yellow, Yellow, Yellow, Yellow with red, Yellow, Pink, Blue |
| Which off the spices (Oregano, Chili Powder, Clove) allows any growth of bacteria? | Chili Powder |
| What microbe was not able to grow on any of the spices? | B. megaterium |
| If you get a green sheen on Eosin Methylene Blue agar, what bacteria have you found? | E. coli |
| MPN indicates what? | Most Probable Number of coliforms in 100ml or water |
| When the P-glo (GFP) is in the presence of arabinose, what is the result? | It will glow under UV light |
| What does GFP stand for? | Green Florescent Protein |
| How does the plasmid get incorporated into the genome of the bacteria? | Heat shock it. Go from cold to hot to cold again. |
| Blank for printing | Blank for printing |