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BKB Micro Lab Final

BIOL2210 study for Lab final

QuestionAnswer
What media is used for Butandiol or Acetoin production (VP)? MR-VP tubes and Vogus-Proskauer reagent A and Barritt's reagent B
What media is used for Hydrogen Sulfide production? SIM Tube
What media is used for Indole production? Tryptone broth with Kovacs reagent
What media is used for Starch Hydrolysis? Starch Agar with Iodine
What media is used for Urease production? Urea Agar
What media is used for Citrate Utilization? Simmons Citrate Agar
What media is used for Casein Hydrolysis? Skim Milk Agar
What media is used for Mixed Acid Fermentation (MR)? MR-VP tubes with Methyl Red
What media is used for Glucose Fermentation? Phenol Red broth with inverted vial
What media is used for Nitrate Reduction? Nitrate broth with Nitrate Reagent A & B
What media is used for Catalase production? Nutrient Agar with H2O2
The appearance of a positive result for Glucose Fermentation without any gas is? Yellow color without any 'air' in the inverted vial
The appearance of a positive result for Citrate Utilization is? Color change from Green to Blue
The appearance of a positive result for Urease production is? Red color
The appearance of a positive result for Indole production is? Red layer on the top of the medium
The appearance of a positive result for Butandiol or Acetoin production is? Red or Pink color
The appearance of a positive result for Hydrogen sulfide production is? A dark color in the SIM tube
The appearance of a positive result for Nitrate reduction is? A red color appears
The appearance of a positive result for Catalase production is? Foams or Bubbles with the addition of H2O2
The appearance of a positive result for Casein production is? Clear zone around the streak
The appearance of a positive result for Starch Hydrolysis is? Clear zone around the streak
The appearance of a positive result for Mixed Acid Fermentation is? Red Color
What does the Gelatin stab testing for? The enzyme Gelatinase
What does the Fluid Thioglycollate Medium (FTM) testing for? If it is aerobic or Anaerobic
What does the motility Medium testing for? Flagella
Why is blood agar used to test for strep? If positive, the bacteria form tiny colonies on this medium
What is the difference between beta-hemolysis and alpha-hemolysis? Beta-hemolysis creates clear zones and indicates Strep Throat where alpha-hemolysis creates tan or light green zones and is not strep throat
What color zone does a gamma-hemolysis create? White
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What is the primary stain in gram staining? Crystal Violet
What is the mordant in gram staining? Iodine
What is the decolorizer in gram staining? Ethanol
What is the counterstain in gram staining? Safranin
What is the process for staining Smear, Heat Fix, Stain
The pure culture techniques use what method? Streak plate method
Using the streak plate method on nutrient agar, what color would the coloniesbe for E. coli? White
Using the streak plate method on nutrient agar, what color would the coloniesbe for S. marcescans? Red
Using the streak plate method on nutrient agar, what color would the coloniesbe for M. luteus? Yellow
How do you determine the effectiveness of a disinfectant? Measuring across the zone of inhibition. The greater the zone, the greater the effectiveness.
To use the Enterotube II, what two conditions must exist? Oxidase Negative and Gram Negative rod
Positive results in the Enterotube II are: Yellow, Purple, Purple, yellow with Black, Yellow, Yellow, Yellow, Yellow, Yellow with red, Yellow, Pink, Blue
Which off the spices (Oregano, Chili Powder, Clove) allows any growth of bacteria? Chili Powder
What microbe was not able to grow on any of the spices? B. megaterium
If you get a green sheen on Eosin Methylene Blue agar, what bacteria have you found? E. coli
MPN indicates what? Most Probable Number of coliforms in 100ml or water
When the P-glo (GFP) is in the presence of arabinose, what is the result? It will glow under UV light
What does GFP stand for? Green Florescent Protein
How does the plasmid get incorporated into the genome of the bacteria? Heat shock it. Go from cold to hot to cold again.
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Created by: brian.belson
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