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Denaturation

Denaturation of Enzymes - Leaving Cert Biology

QuestionAnswer
What is the First step in the Denaturation experiment? Place some celery stalks in a blender and add some water, then blend to form a pulp.
Why is Celery used in the experiment? Celery is used as it contains the enzyme Catalase which is the enzyme for this experiment.
Second step of the Denaturation experiment? Filter pulped celery into a beaker using coffee/filter paper.
Why does the Celery need to be filtered? It removes cell parts.
3rd step of the Denaturation experiment? Place some of the filtrate into a large graduated cylinder and place in warm water bath at 25 degrees Celsius.
The fourth step of the Denaturation experiment? Add pH buffer 9 to this solution to the graduated cylinder.
What does the pH buffer ensure? It ensure that there is no change in pH during the experiment, hence allowing there to be a controlled variable.
What is the 5th step of the Denaturation experiment? Using a dropper add one drop of washing-up liquid. the washing-up liquid traps any oxygen produced.
6th step of the Denaturation experiment? Add some Hydrogen Peroxide (H2O2) to a smaller graduated cylinder and then place into the same water bath as the graduated cylinder containing the celery solution.
Seventh step of the Denaturation experiment? Leave both solutions in the water bath until they reach 25 degrees Celsius.
The eighth step of the Denaturation experiment? Add the H2O2 to the celery solution.
What is the 9th step of the Denaturation experiment? Note if foam is produced or not after 2 minutes.
What is the tenth step of the Denaturation experiment? Boil some of the celery solution for 10 minutes.
What happens after step 10? Steps 3-9 are repeated with the boiled celery solution.
What is the purpose of the boiled catalase solution? It acts as a control for the experiment.
Created by: AW117
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